摘要
目的:探讨过氧化物酶体增殖物激活型受体γ(PPARγ)及其配体(15-脱氧-前列腺素J2,15-d-PGJ2)对细胞滋养细胞表达基质金属蛋白酶-2(MMP-2)和MMP-9的调控作用。方法:采用免疫荧光细胞化学方法检测细胞滋养细胞中PPARγ的表达;利用免疫荧光共聚焦技术观察15-d-PGJ2作用前后细胞滋养细胞MMP-2和MMP-9表达强度的变化;通过荧光定量PCR(Real-timePCR)和Westernblot方法定量检测MMP-2和MMP-9mRNA和蛋白的表达变化。结果:在细胞滋养细胞中有PPARγ蛋白表达,且主要定位在细胞滋养细胞核中;15-d-PGJ2作用后细胞滋养细胞中MMP-2和MMP-9的表达明显下降,与对照组相比差异显著(P<0.01);15-d-PGJ2对MMP-2的作用强于MMP-9。结论:PPARγ及其配体15-d-PGJ2调节滋养细胞浸润作用可能是通过调节MMP-2和MMP-9的表达实现的。
Objective： To investigate the influence ofperoxisome proliferator-activated receptor gamma （PPARγ） and its ligand 15-deoxy-delta（12,14）-prostaglandin J2（15-d-PGJ2） on the expression of matrix metalloproteinase（MMP）-2 and MMP-9 in cytotrophoblast in first trimester. Methods： The expression of PPARγ in cytotrophoblast in first trimester was examined by hianunocytochemistry, and the regulation of PPARγ ligands 15-d-PGJ2 on the expression of MMP-2 and MMP-9 in cytotrophoblast was examined by confocal technique, Real-time quantitative PCR and Western blot. Results： PPARγ expression was detectable in the nucleus of cytotrophoblast cell. The expressions of MMP-9 and MMP-2 in cytotrophoblast cell were inhibited by 15-d-PGJ2 sisitificantly （P〈0.01）, and in a concentration-dependent manner. Effect of 15-d-PGJ2 on the expression of MMP-2 was higher than that on MMP-9. Conclusion： PPARγ ligand 15-d-PGJ2 can inhibit cytotrophoblast invasion through downregulating expressions of MMP-2 and MMP-9.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2007年第2期107-112,共6页
Reproduction and Contraception
