期刊文献+

环氧合酶-2在高糖刺激的系膜细胞和糖尿病肾病模型大鼠肾皮质的表达及意义 被引量:3

Expressions of COX-2 in mesangial cells stimulated by high glucose and renal cortex in rats with diabetic nephropathy and its significance
下载PDF
导出
摘要 目的探讨环氧合酶-2(COX-2)与糖尿病肾脏病变的关系及在糖尿病肾病发病中的意义。方法Westernblot检测了STZ诱导的糖尿病肾病模型大鼠(DN模型组)肾皮质COX-2的表达,以正常大鼠(对照组)肾皮质做对照;并检测了高糖(HG组)及前列腺素E2(PGE2组)刺激的大鼠肾小球系膜细胞COX-2表达,同时还检测了HG组和PGE2组分别刺激及共同刺激(HG+PGE2组)后肾小球系膜细胞的总蛋白量,以正常培养的大鼠肾小球系膜细胞(NG组)作为对照。结果HG组COX-2蛋白表达较NG组明显增加(P<0.01);PGE2也可诱导COX-2的表达增加(P<0.01)。STZ组大鼠肾脏COX-2表达均较CTR组增加(P<0.01);HG组和PGE2组细胞内总蛋白量均较NG组增高(P<0.05),HG+PGE2组细胞内总蛋白量较NG组明显增加(P<0.01),HG+PGE2组与单纯PGE2刺激组比较也增加(P<0.05)。结论COX-2在高糖和PGE2刺激的肾小球系膜细胞及糖尿病肾病大鼠肾皮质的表达增加,高糖和PGE2可导致肾小球系膜细胞肥大,COX-2可能是导致糖尿病早期肾脏肥大的主要因素之一。 Objective To explore the significance of relationship between COX-2 and diabetic nephropathy (DN) and its role in the onset of DN. Methods The expressions of COX-2 protein in renal cortex of diabetes rats induced by STZ were detected by Western blot, renal cortex of normal rats as control. The expression of COX-2 protein in mesangial cells induced by high glucose (HG) or COX-2 major metabolite PGE2 were deteted by Western blot. Furthermore, the total protein content in mesangial cells induced by HG, PGE2, HG + PGEz stimulation was mesasured. Results COX-2 protein expression was markedly increased in HG group and PGEz group compared with that of controls(P〈0.01 ). The kidney hypertrophy index and COX-2 expression in the cortical tissue were significantly increased in STZ group compared with those in control group( P 〈 0.01 ). The total protein content was significantly increased in HG and PGE2 group compared with that in control group(P 〈0.05). The total protein content was significantly increased in HG +PGE2 group compared with that in control and PGE2 group(P〈0.05). Conclusions COX-2 expression is increased in mesangial cells stimulated by HG and renal cortex in DN rats. COX-2 might be one of important factors related to the pathogenesis of renal hypertrophy in eraly DN.
出处 《中国老年学杂志》 CAS CSCD 北大核心 2007年第4期320-322,共3页 Chinese Journal of Gerontology
关键词 环氧合酶-2 糖尿病肾病 系膜细胞 Cyclooxygenase-2 Diabetic nephropathy Mesangial cell
  • 相关文献

参考文献6

二级参考文献34

  • 1Parving HH, Osterby M, Ritz E. Diabetic Nephropathy[A]. In: Brenner BM ed. The Kideny[M]. Ioth ed.Philadalphia: WBS aunders company, 2000.1731~1754.
  • 2Tjandrawinata RR, Dahiya R, Hughes M. Induction of cyclooxygenase-2 mRNA by prostaglandin E2 in human prostatic carcinoma cells[J]. Br J Cancer, 1997, 75(8): 1111~1118.
  • 3Curtis SP, Ng J, Yu Q, et al. Renal effects of etoricoxib and comparator nonsteroidal anti-inflammatory drugs in controlled clinical trails[J]. Clin Ther, 2004, 26(1): 70~83.
  • 4Sandhu GK, Heyneman CA. Nephrotoxic potential of selective cyclooxygenase-2 inhibitors[J]. Ann Pharmacother, 2004, 38(4): 700~704.
  • 5Tomasoni S, Noris M, Zappela S, et al. Upregulation of renal and systemic cyclooxygenase-2 in patients with active lupus nephritis[J].J Am Soc Nephrol,1998, 9:1202~1212.
  • 6Takano T, Cybulsky AV, Cupples WA, et al. Inhibition of cyclooxygenases reduces complement-induced glomerular epithelial cell injury and proteinuria in passive Heymann nephritis[J]. J Pharmacol Exp Ther, 2003, 305(1): 240~249.
  • 7Yang T, Singh I, Pham H, et al. Regulation of cyclooxygenase expression in the kidney by dietary salt intake[J]. Am J Physiol, 1998, 274:F481~F489.
  • 8Knmhoff M, Jeck DM, Seyberth W, et al. Cyclooxgenase-2 expression is associated with the renal macula densa of patients with Bartter-like syndrome[J]. Kidney Int, 2000, 58:2420~2424.
  • 9Fujihara CK, Antunes GR, Mattar AL, et al. Cyclooxygenase-2 (COX-2) inhibition limits abnormal COX-2 expression and progressive injury in the remnant kidney[J]. Kidney Int, 2003, 64(6): 2172~2181.
  • 10Komers R, Lindsley JN, Oyama TT, et al. Immunohistochemical and functional correlations of renal cyclooxygonase-2 in experimental diabetes[J]. J Clin Invest, 2001, 107(7): 889~898.

共引文献406

同被引文献51

  • 1李正军,刘国安.糖尿病肾病相关基因的研究进展[J].甘肃科技,2005,21(6):143-145. 被引量:2
  • 2陈芳,孙世澜,孙汉英.环氧化酶-2抑制剂对糖尿病大鼠肾脏活性作用的研究[J].中国血液流变学杂志,2005,15(2):185-190. 被引量:7
  • 3刘志纯,周巧玲,彭卫生,邓声莉.糖尿病大鼠肾脏Cox-2表达及洛沙坦对其影响的实验研究[J].中国医师杂志,2005,7(11):1457-1460. 被引量:3
  • 4邹丽霞,谷卫.环氧化酶-2与糖尿病[J].国际内分泌代谢杂志,2006,26(1):45-47. 被引量:11
  • 5Boulton AJ, Vinik AI, Arezzo JC, et al. Diabetic neuropathies:a statement by the American Diabetes Association.Diabetes Care, 2005, 28: 956-62.
  • 6Singleton JR, Smith AG, Russell J, et al. Polyneuropathywith impaired glucose tolerance: implications fordiagnosis and therapy. Curr Treat Opt Neurol, 2005, 7: 33-42.
  • 7Edwards JL, Vincent AM, Cheng HT, et al. Diabeticneuropathy: mechanisms to management. Pharmacol Ther,2008, 120: 1-34.
  • 8Bujalska M, Tatarkiewicz J, Corde de A, et al. Effect ofcyclooxygenase and nitric oxide synthase inhibitors onstreptozotocin induced hyperalgesia in rats. Pharmacology,2008, 81: 151-7.
  • 9Cheng HT, Dauch JR, Su S, et al. p38 mediates mechanicalallodynia in a mouse model of type 2 diabetes. Mol Pain,2010, 6: 28-42.
  • 10Amaya F, Samad TA, Barrett L, et al. Periganglionicinflammation elicits a distally radiating pain hypersensitivityby promoting COX-2 induction in the dorsal rootganglion. Pain, 2009, 142: 59-67.

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部