摘要
目的探讨胃癌患者外周血淋巴细胞CD8、CD28的表达及其临床意义。方法用流式细胞术对48例胃癌患者和30例健康体检者外周血淋巴细胞CD8及CD28进行了检测。结果(1)胃癌组CD8+CD28-细胞与对照组比较明显增高(P<0.05),CD8+CD28+细胞则明显减低(P<0.01)。(2)胃癌患者手术后CD8+CD28-细胞明显低于手术前(P<0.01),CD8+CD28+细胞明显高于手术前(P<0.05)。(3)胃癌患者淋巴结转移组CD8+CD28-细胞明显低于未转移组(P<0.01)。(4)Ⅲ期胃癌患者CD8+CD28-细胞与Ⅰ期和Ⅱ期相比明显增高(分别P<0.01,P<0.05),随着临床分期的进程CD8+CD28+细胞、CD8-CD28+细胞逐渐减低(分别P<0.01,P<0.05)。结论检测胃癌患者的外周血中的CD8和CD28的表达,对了解患者的免疫状况,疾病进展和指导临床对患者进行免疫调节治疗方面有一定的价值。
Objective To clear about the significance of CD8 and CD28 expressions on lymphocytes of peripheral blood in patients with gastric cancer. Methods The expressions of CD8 and CD28 on T lymphocytes of peripheral blood were determined from 48 patients with gastric cancer and 30 healthy donors by flow cytometry. Results ①The level of CD8^＋CD28^-expression of the patient group was remarkably higher that of healthy donors（P 〈 0.05）, but the CD8^＋CD28^- expression significantly lower than that of healthy group（P 〈 0.01）. ② The expression of CD8^＋CD28^- T cells in the postoperation patients were remarkably lower（P 〈 0.01）, and CD8^＋CD28^＋expression was significantly higher（P 〈 0.05） compared with that of preoperation. ③The levels of CD8^＋CD28^＋ expression on peripheral blood lymphocytes in patients with lymph node metastasis were in evidence lower than that of the patients without the metastasis （P 〈 0.01）. ④The amounts of CD8^＋CD28^- ceils in m stage patient group were higher than those in Ⅰ and Ⅱ stage groups （P 〈 0.01, P 〈 0.05 respectivly） and with advance of clinic classification, the amounts of CD8^＋CD28^＋ and CD8^-CD28^＋ lymphocytes were decreasing（P 〈 0.01, P 〈 0.05 respectivly）. Conclustion Detecting the experessions of CD8 and CD28 in peripheral blood lymphocytes was of importance to acquire the immune conditions and the complaint advance of patients with gastric cancer and to adjust the patient＇s immune function.
出处
《江西医学检验》
2007年第1期17-18,54,共3页
Jiangxi Journal of Medical Laboratory Sciences
