摘要
目的观察异丙酚对幼兔未成熟心肌缺血再灌注损伤的影响,初步探讨其作用机制。方法24只日本大耳幼兔(雌雄不限,兔龄21～28d)随机分为缺血再灌注组(A组)、异丙酚组(B组)和对照组(C组)3组。结扎冠状动脉前降支(LAD)制作在体兔心肌缺血再灌注损伤动物模型,其中C组只穿线不结扎,B组于结扎前给予异丙酚300μg·kg-1·h-1,A、B组结扎LAD60min,再灌注120min时抽取动脉血测血清一氧化氮(NO)、丙二醛(MDA)和同型半胱氨酸(Hcy)浓度,取缺血区心肌测心肌含水量,透射电镜观察缺血区心肌超微结构。结果与C组比较,A组血清NO浓度[(246.04±11.65)μmol/L]、MDA浓度[(10.20±1.90)nmol/L]、Hcy浓度[(18.62±6.33)μmol/L]和心肌含水量[(77.26±0.42)%]均升高(P<0.05),B组血清NO浓度[(280.00±13.23)μmol/L]升高(P<0.05)。与A组比较,B组血清NO浓度[(280.00±13.23)μmol/L]升高,MDA浓度[(8.00±1.17)nmol/L]、Hcy浓度[(10.54±3.76)μmol/L]和心肌含水量[(74.16±0.28)%]下降(P<0.05)。电镜观察显示,B组心肌超微结构损伤程度轻于A组,C组心肌结构接近正常。结论异丙酚对幼兔心肌缺血再灌注损伤有保护作用,其机制与异丙酚增加心肌的NO浓度、减少MDA发挥其抗氧化作用及减轻Hcy堆积等有关。
Objective To investigate the effects of Propofol on rabbit immature myocardium which have underwent MIRI. Methods Twenty-four rabbits either sexes aged 21-28 days weighing 300-350 g were randomly divided into three groups （n=8 eaeh）：Ischemia-reperfusion group （A group）, propofol group （B group） and control group （C group）. In three groups, left common carotid artery was cannulated for blood collection. Chest was opened and myocardial ischemia was induced by occlusion of anterior descending branch of left coronary artery and confirmed by cyanosis of local myocardial and by elevation or depression of S-T segment and/or high T wave. LAD was not occlused in group C and rabbits were pretreated by propofol. Blood samples were taken for detection of serum NOS activity and plasma NO,MDA,Hcy concentration. At the end of experiment, myocardial tissue was obtained from ischemia area for water-content and electron microscope examination. Results To compare with C group, the serum NOS activity and NO concentration were significantly higher（P〈0.05）in group B. In group B, the serum Hcy and MDA concentration were significantly lower than those in A group（P〈0.05） and the serum NOS activity and NO concentration were significantly higher than those in A group （P〈0.05）. Electron microscope examination showed that ultrastructure of cardiac myocytes was severely damaged in A group. In B group, the damage was milder as compared with that in group A. In group C, the ultrastructure of cardiac myocytes was close to normal. Conclusion Propofol has protective effects on myocardium with ischemical reperfusion injury to some degree and the mechanism of protection is related to anti-oxidation, promotion of NOS activity and reducing the concertration of Hcy.
出处
《中国心血管病研究》
CAS
2007年第3期221-223,共3页
Chinese Journal of Cardiovascular Research
