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4种氟喹诺酮类药物在肉鸡组织中残留的研究 被引量:21

Simultaneous Determination of Four Fluoroquinolone Residues in Broilers Tissues
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摘要 采用高效液相色谱法同时检测肉鸡肌肉、脂肪、肝脏和肾脏组织中4种氟喹诺酮类药物(FQS)的残留。检测条件为:采用HP-StableBond C18分析柱(5um,250mm×4.6mm),以15mmol/L的四丁基溴化铵溶液-乙腈(体积比94:6)为流动相(用乙酸钠缓冲液调pH3.6),流速1.0ml/min,柱温30℃;采用荧光检测器检测,激发波长280nm,发射波长460nm。测定结果表明,该方法对FQS的最低检测限为0.92ug/kg,在5.00~100.00ug/mg的线性范围内,溶液含量与峰面积的相关系数达0.9998以上。在高、中、低3种含量水平下对所测肉鸡组织中4种FQS进行回收率测定,结果为74%~99%,相对标准偏差低于6.5%,该方法能同时测定4种FQS在动物组织中的残留,具有简便、准确、灵敏度较高的特点,符合痕量测定的要求。 An high performance liquid chromatographic method with a fluorescence detector was established to determine four fluoroquinolone residues in broilers tissues. The chromatographic conditions were as follows: HP- StableBond C18 column (5 um,250 mm×4.6 mm) was used at 30℃ with 15 mmol//L tetrabutylammonium bromide solution (pH3.0)- acetonitrile (94:6,v/v) as the mobile phase with a flow rate of 1.0 ml/min, At the excitation wavelength of 280 nm and emission wavelength of 460 nm . The detection limit for broilers tissues was 0. 92 ug/kg. The linear range was from 5.00-100.00 ug/mg, And correlation coefficients were more than 0.9998. At the levels of 10, 50 and 100 ug/kg, The recoveloped can meet the requirement for residue analysis.
作者 刘波静
出处 《中国畜牧兽医》 CAS 2007年第1期112-115,共4页 China Animal Husbandry & Veterinary Medicine
关键词 高效液相色谱法 肉鸡 环丙沙星 氧氟沙星 诺氟沙星 恩诺沙星 high performance liquid chromatograpy broilers ciprofloxacin ofloxacin norfloxacin enrofloxacin
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  • 1An W,Guo G,Su D,et al.Analysis and Testing Technology and Instruments,2004,10(2):107.
  • 2Johnston L,Mackay,C Roft M.J Chromatogr A,2002,982(1):97.
  • 3Marazuela M D,Moreno-Bondi M C.J Chromatogr A,2004,1034(1~2):25.
  • 4Nadon A,Martinez-Larranaga.Pharamacokinetics and residues of enrofloxacin in chickens[J].Am J Vet Res,1995,56(4):501~506.
  • 5Schneider M J,Donoghue D J.Anal Chim Acta,2003,483(1~2):39.

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