摘要
自疑似大肠杆菌病例中分离到大肠杆菌44株,并对其病原特性进行了研究,结果表明这些菌的生化特性基本一致,与文献报道相符。对此44株大肠杆菌进行血清型鉴定,鉴定出血清型39株,其中O血清型13种,以O78、O35、O24、O1、O15、O76和O88等7个血清型为主,占定型菌株的84.6%。其中血清型为O78的菌株占定型菌株的38.5%,为优势血清型。用9种抗菌药物(头孢唑啉、庆大霉素、阿米卡星、链霉素、环丙沙星、诺氟沙星、青霉素、妥布霉素、红霉素)进行了药敏试验,结果表明分离菌株呈现出不同程度的耐药性,其中青霉素、红霉素的耐药率最高(均为97.7%),其次是环丙沙星(81.8%),大多数为多重联合耐药;而阿米卡星、妥布霉素和庆大霉素的敏感率较高,其中以阿米卡星(90.9%)最敏感。
Fourty-four strains of escherichia coli from chickens were isolated from cases that were suspected to be escherichia coli, and the pathogen＇s characteristics were identified. It was found that the biochemical characters of these bacteria were fundamentally identical and agreed with documents. Moreover, the serotypes of 39 isolates were identified. Thirteen types of serum O were found out, among which O78,O35 ,O24 ,O1,O15 ,O76 and O88 were main serotypes with a 84.6% of all the isolates. The dominant serotype of these isolates was O78 with a 38.5 %of all the isolates. Antibiotic resistance of 44 strains escherichia coli to nine antimicrobial drugs （Cefazolin, Gentamicin, Amikacin, streptomycin, Ciprofloxacin, Norfloxacin, Penicillin, Tobramycin,Erythromycin） was detected and analyzed by disk diffusion susceptibility test. The results indicated that the isolates showed a greater or lesser degree resistance to nine antimicrobial drugs. Most isolates showed resistance to multiple anti- biotics and displayed a highest level resistance to Penicillin and Erythromycin with a frequency of 97.7 %, followed by Ciprofloxacin with a frequency of resistance of 81.8%. While most isolates displayed a higher level susceptibility to Amikacin, Tobramycin and Gentamicin,especially to Amikacin with a frequency of 90.9 %.
出处
《中国畜牧兽医》
CAS
2007年第2期119-121,共3页
China Animal Husbandry & Veterinary Medicine
关键词
鸡
大肠杆菌
分离鉴定
血清型
药敏试验
chicken
escherichia coli
separation and appraisal
serotype
drugs susceptibility test
