摘要
目的:为了制备转染效率高的新型基因载体,本实验制备由聚酰胺-胺型树状大分子(PAMAM)与聚乳酸/乙醇酸共聚物(PLGA)复合的新型基因载体,并评价了其转染效率。方法:采用溶剂挥发法制备了PAMAM-PLGA新型基因载体,并且通过考察纳米基因载体粒径,粒子形态,稳定性,zeta电位、pH值等,从而确定了最佳的制备工艺。用电泳实验和绿色荧光蛋白标记方法研究了它的转染效率。结果:新型基因载体能够很好的携带DNA进入细胞。结论:在适合的制备工艺条件下可以制备出粒径大约100nm,分散系数为0.088的新型纳米基因载体,其转染效率要高于单纯的PAMAM。
OBJECTIVE To preparation higher transfer efficiency gene delivery, we prepared PAMAM-PLGA new nanoparticles gene delivery and evaluated their transfer efficiency. METHODS PAMAM-PLGA nanoparticles were prepared by solvent evaporation method. By investigating nanoparticles size and distribution, nanoparticles morpHology, nanoparticles stability, pH and zeta potential,the relations between properties and synthetic process were studied and found out a set of desired process conditions. We used gel retardation assay experiment and green fluorescence protein marker to investigate their transfer efficiency. The result indicated that the new gene delivery can carry DNA into cells. RESULTS The result indicated that the new gene delivery can carry DNA into cells. CONCLUSION Higher transfer efficiency gene delivery PAMAM-PLGA nanoparticles were prepared by solvent evaporation method. The nanoparticles size was about 100nm and PDI was 0.088. The transfer efficiency of PAMAM-PLGA was higher than pure PAMAM.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第2期202-205,共4页
Chinese Journal of Hospital Pharmacy
