摘要
以球形期的大豆体细胞胚为转基因受体，以抗性体细胞胚筛选率为转化率指标，用农杆菌介导法进行遗传转化，对转化的最佳条件进行了初步研究。结果表明：大豆体细胞胚预培养2d，菌液浓度OD600=0．5-0．7，AS浓度为50，100μmol/L，侵染时间10min，共培养时间2-3d有利于提高转化率；对不同筛选代数观察结果表明，转化率在3个月以前较低，而在3个月以后则基本稳定在8％。
The factors influencing genetic transfomaation of somatic embryos of soybean mediated by Agrobacter/um tumefaction were studied. The optimum conditions were as followed： injured treatment of somarie embryos of soybean, two-day pre-culture, a bacterial concentration of 0.5 - 0.7 （OD600）, 10 minutes of infection,50 - 100 μmol/L AS. The transformation frequency decreased with the delay of the date of selection in three months and the transformation frequency kept at a 8 % level after three months.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2007年第1期44-47,60,共5页
Journal of Jilin Agricultural University
基金
"国家植物转基因技术研究开发与中试基地建设"专项基金资助项目(J99-B-001)
关键词
大豆
农杆菌介导
体细胞胚
遗传转化
soybean
mediated by Agrobacterium
somatic embryos
genetic transformation
