摘要
目的：探讨不同感染度日本血吸虫小鼠肝组织内可溶性虫卵抗原（ＳＥＡ）及其抗体水平和虫卵肉芽肿病变的动态变化与相互关系。方法：免疫组化酶标法和图像分析法。结果：感染后４ｗｋ，３组小鼠肝内均检出ＳＥＡ，此后ＳＥＡ水平逐渐升高，６－９ｗｋ时达高峰，以后逐渐下降。感染后６ｗｋ起，在虫卵周围检出抗ＳＥＡ抗体，８ｗｋ时抗体水平升高，１０ｗｋ后达高峰。３组小鼠在感染后相应时间，肝内ＳＥＡ及抗体水平基本相同。３组小鼠肝内虫卵肉芽肿至感染后５ｗｋ时尚未形成，６－７ｗｋ时虫卵肉芽肿反应最强，其平均直径和面积达最大值，８ｗｋ后逐渐减小。３组小鼠间无显著性差异。结论：肝组织内ＳＥＡ及其抗体水平与虫卵肉芽肿病变密切相关，与感染度无显著关系。
Abstract AIM:To explore the kinetics of soluble egg antigen (SEA) and its antibody level as well as egg granuloma in the liver tissue of mice infected with Schistosoma japonicum of various intensity and their interrelations. METHOD: Immunohistochemical technique and profile analytical technique. RESULTS: In three groups of mice infected with 10, 20 and 40 cercariae, respectively, SEA in the liver tissue was first detected at 4th week post infection (p.i.), reaching a peak level at 6 9th week, and then gradually dropped. Anti SEA antibody in the liver tissue was first detected at 6th week p.i., and then gradually rose to peak level at 10th week p.i., The levels of antigen and antibody were alike correspondingly among the three groups at the same stage after infection. No egg granuloma could be found until 6th week p.i.. The peak of the average diameter and area of liver egg granuloma was noted at 7th week p.i., then dropped gradually, showing no significant differences among the three groups. CONCLUTION: The levels of SEA and its antibody in the liver tissue were closely correlated with the egg granuloma, and showed no significant correlation with the intensity of the infection. Key works: Schistosoma japonicum, intensity of infection, antigen, antibody, egg granuloma
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1996年第3期193-196,共4页
Chinese Journal of Parasitology and Parasitic Diseases
