摘要
目的探讨罗格列酮对2型糖尿病(T2DM)OLETF大鼠胰岛β细胞凋亡的影响。方法OLETF大鼠40只,随机分为罗格列酮干预组和对照组各20只,LETO大鼠10只为正常对照组。干预组从8周龄起以罗格列酮3mg·kg-1·d-1灌胃,其余两组以等量清水灌胃。定期行OGTT,分批宰杀,HE染色观察胰岛结构,TUNEL+免疫组化法检测β细胞凋亡率。结果干预组高血糖出现延迟,程度减轻,胰岛结构明显好于对照组。在32和40周龄时对照组β细胞凋亡率分别为0·28%±0·03%、0·26%±0·03%,干预组为0·18%±0·02%、0·17%±0·04%。同周龄干预组β细胞凋亡率显著低于对照组(P<0·01)。结论罗格列酮能显著降低OLETF大鼠β细胞凋亡率。
Objective To investigate the effects of rosiglitazone on β-cell apoptosis in OLETF rats. Methods 40 male OLETF rats were divided randomly into rosiglitazone group （Group RSG, n=20） and control group （Group control, n = 20）. 10 male LETO rats were used as normal control （Group LETO）. Rats of group RSG were given rosiglitazone（3 mg·kg^-1·d^-1 ） intragastrically since age of 8 weeks. OGTT blood glucose was determined. The morphological change of pancrease and β- cell apoptosis were determined. Results Hyperglycemia and morphological change of pancreas were mild in group RSG. The percentage of β-cell apoptosis at age of 32 and 40 weeks were 0. 18±0.02%, 0. 17±0. 04% in group RSG; 0. 28% ±0. 03%, 0. 26%±0.03% in group control respectively. The percentage of B-cell apoptosis of group RSG was significantly lower than that of group control at age of 32 and 40 weeks（P〈0. 01）. Conclusions Rosiglitazone can decrease β-cell apoptosis in OLETF rats.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2007年第1期44-46,共3页
Chinese Journal of Diabetes
基金
国家自然科学基金资助项目(30371839)
广东省科技计划基金资助项目(社会发展攻关2003C34424)
广东省科技计划基金资助项目(2004B30701011)
关键词
糖尿病
2型
凋亡
罗格列酮
Diabetes mellitus, type 2
Apoptosis
Rosiglitazone
