摘要
目的:克隆人IL-24基因并构建真核表达载体,转染Ca Ski细胞进行真核表达.方法:利用RT-PCR技术扩增出Ca Ski细胞中的IL-24基因.将IL-24基因克隆入pcDNA3.1(+)中,构建真核表达质粒pcDNA3.1(+)-hIL-24.将重组质粒转染入CaSki细胞,利用Western blot检测hIL-24的表达.结果:PCR及测序结果均证实成功克隆了人IL-24基因,构建了真核表达质粒pcDNA3.1(+)-hIL-24,该质粒被转染入Ca Ski细胞中能表达出hIL-24蛋白.结论:成功构建了hIL-24基因的真核表达载体.
AIM： To clone human IL-24 （ hIL-24 ） gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. METHODS： hIL-24 gene was acquired by RT-PCR from the total RNA of Ca Ski cell and cloned into plasmid pcDNA3. 1 （ ＋ ） to generate the eukaryotic expression vector named pcDNA3. 1 （ ＋ ）-hIL-24. pcDNA3. 1 （ ＋ ）-hIL-24 was transfected into Ca Ski cells to express hIL-24 protein. RESULTS： PCR and sequencing revealed that hIL-24 gene was successfully cloned into plasmid pcDNA3.1 （ ＋ ） and its eukaryotic expression vector was constructed. The vector was able to express hIL-24 protein after transfected into Ca Ski cells. CONCLUSION： The hIL-24 eukaryotic expression vector has been successfully constructed.
出处
《第四军医大学学报》
北大核心
2007年第5期469-471,共3页
Journal of the Fourth Military Medical University
基金
重庆市教委重点项目(渝教科[2001]12号)
