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Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria 被引量:1

Isolation and preliminary characterization of a 3-chlorobenzoate degrading bacteria
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摘要 A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several (halo)aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no (chloro)catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway. A study was conducted to compare the diversity of 2-, 3-, and 4-chlorobenzoate degraders in two pristine soils and one contaminated sewage sludge. These samples contained strikingly different populations of mono-chlorobenzoate degraders. Although fewer cultures were isolated in the uncontaminated soils than contaminated one, the ability of microbial populations to mineralize chlorobenzoate was widespread. The 3- and 4-chlorobenzoate degraders were more diverse than the 2-chlorobenzoate degraders. One of the strains isolated from the sewage sludge was obtained. Based on its phenotype, chemotaxonomic properties and 16S rRNA gene, the organism S-7 was classified as Rhodococcus erythropolis. The strain can grow at temperature from 4 to 37℃. It can utilize several (halo)aromatic compounds. Moreover, strain S-7 can grow and use 3-chlorobenzoate as sole carbon source in a temperatures range of 10-30℃ with stoichiometric release of chloride ions. The psychrotolerant ability was significant for bioremediation in low temperature regions. Catechol and chlorocatechol 1,2-dioxygenase activities were present in cell free extracts of the strain, but no (chloro)catechol 2,3- dioxygenase activities was detected. Spectral conversion assays with extracts from R. erythropolis S-7 showed accumulation of a compound with a similar UV spectrum as chloro-cis,cis-muconate from 3-chlorobenzoate. On the basis of these results, we proposed that S-7 degraded 3-chlorobenzoate through the modified ortho-cleave pathway.
出处 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2007年第3期332-337,共6页 环境科学学报(英文版)
关键词 chlorobenzoate BIODEGRADATION Rhodococcus erythropolis psychrotrophic bacteria PATHWAY chlorobenzoate biodegradation Rhodococcus erythropolis psychrotrophic bacteria pathway
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  • 1Adriaens P,Focht D D,1991.Continuous co-culture degradation of selected polychlorinated biphenyl congeners by Acinetobacter spp.in an aerobic reactor system[J].Environ Sci Technol,24:1042-1049.
  • 2Ajithkumar P V,Kunhi A A,2000.Pathways for 3-chloro-and 4-chlorobenzoate degradation in Pseudomonas aeruginosa 3mT[J].Biodegradation,11:247-261.
  • 3Bell K,Philip J,Aw D W et al.,1998.The genus Rhodococcus[J].J Appl Microbiol,85:195-210.
  • 4Bernard J,van der Woude,Jan G et al.,1996.Extent of reductive dechlorination of chlorobenzoates in anoxic sediment slurries depends on the sequence of chlorine removal[J].Environ Sci Technol,30:1352-1357.
  • 5Bollag J M,Briggs G G,Dawson J E et al.,1968.2,4-D metabolism:enzymatic degradation of chlorocatechols[J].J Agfic Food Chem,16:829-833.
  • 6Bott T L,Kaplan L A,2002.Autecological properties of 3-chlorobenzoate-degrading bacteria and their population dynamics when introduced into sediments[J].Microb Ecol,43:199-216.
  • 7Brenner V,Rucka L,Totevova S et al.,2004.Efficiency of chlorocatechol metabolism in natural and constructed chlorobenzoate and chlorobiphenyl degraders[J].J ApplMicrobiol,96:430-436.
  • 8Brunsbach F R,Reineke W,1993.Degradation of chlorobenzoates in soil slurry by special organisms[J].Appl Microbiol Biotechnol,39:117-122.
  • 9de Carvalho C C,da Fonseca M M,2005.The remarkable Rhodococcus erythropolis[J].Appl Microbiol Biotechnol,67:715-726.
  • 10de Wever H,de Cort S,Noots I et al.,1997.Isolation and characterization of Rhodococcus rhodochrous for the degradation of the wastewater component 2-hydroxybenzothiazole[J].Appl Microbiol Biotechnol,47:458-461.

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