摘要
为了研究ｐ５３和ｋｉ－ｒａｓ基因在苯并芘致肺癌过程中的作用，我们用免疫组织化学，Ｎｏｒｔｈｅｒｎ杂交和ＰＣＲ－ＳＳＣＰ方法对人肺癌细胞系（ＢＴ）ｐ５３基因和ｒａｓ基因进行了研究。结果发现ＢＴ癌细胞中有ｐ５３蛋白和ｍＲＮＡ水平增高，ｃｏｄｏｎ２７３出现了Ｇ→Ｔ突变；ｐ２１癌蛋白高表达，Ｋｉ－ｒａｓ基因ｍＲＮＡ水平增高和ｃｏｄｏｎ１２出现Ｇ→Ｔ突变。结果提示二者协同参与了苯并芘致肺癌过程。从分子水平为苯并芘的致肺癌变机制研究提供了证据。
In order to determine the effect of p53 and Ki ras genes on the carcinogenesis of human lung induced by benzo(a)pyrene,p53 and Ki ras genes were studied in the human lung carcinoma cell line(BT) derived from BT cancer with the immunohistochemistry method,Northern blot analysis and PCR SSCP.In the BT carcinoma cells,p53 protein and p53 mRNA also overexpressed,G→T mutation was found at coden 273 of p53,p21 oncoprotein and Ki ras mRNA were overexpressed.G→T mutation was found at coden 12 of Ki ras gene.These results showed that p53 and Ki ras gene might play an important role in the carcinogenesis of lung caused by benzo(a)pyrene.This result afforded a proof for studying the mechanism of the carcinogenesis of human lung carcinoma induced by benzo(a)pyrene in the aspect of molecular biology,and consisted with the hypothesis that multiple genetic changes involving both oncogene and tumor suppressor gene during lung carcinogenesis.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1996年第6期244-247,共4页
Journal of Environment and Health
基金
国家自然科学基金
