摘要
目的:建立三叶保健吸品主要成分山奈酚、异鼠李素、槲皮素和甜菜碱的高效液相色谱法(HPLC)含量测定方法。方法:山奈酚、异鼠李素和槲皮素测定:hypersil C18(250 mm×4.6 mm,5μm)色谱柱,流动相甲醇-0.4%磷酸溶液(60∶40,V∶V),检测波长360 nm,流速1.2 ml.min-1。甜菜碱测定:hypersil C18(250mm×4.6 mm,5μm)色谱柱,流动相50 mmol/L KH2PO4(pH4.45,含0.3%辛烷磺酸钠),检测波长200 nm,流速0.7 ml.min-1。结果:山奈酚、异鼠李素、槲皮素和甜菜碱在0.2-4.8μg.ml-1,0.1-2.4μg.ml-1,0.025-0.8μg.ml-1和1.0-32.0μg.ml-1范围内线性关系良好。方法精密度分别为1.5%,2.1%,1.6%和2.2%。三叶保健吸品中山奈酚、异鼠李素、槲皮素和甜菜碱含量分别为0.002 42%,0.000 849%,0.000 249%和0.00636%。结论:本研究所建方法简便,快速,准确,适用于三叶保健吸品主要成分含量测定,可用于其质量控制。
Objective： To establish the HPLC method for determination of the major components （campherol, isorhamnetin, meletin and betaine） in the Sanye Health Cigarette. Methods： Campherol, isorhmnetin and meletin were separated by a Hypersil-C18 column （250 mm × 4.6 mm, 5μm）, and eluted by mobile phase of methanol-0.4 % phosphoric acid （60：40,V：V） and detected at wave length of 360 nm and flow rate of 1.2 ml·min^-1. Betaine was sepa- rated by a Hypersil-C18 column（250 mm × 4.6 mm, 5μm）, eluted by mobile phase of 50 mM potassium dihydrogen phos- phate（pH4.45, containing 0.3% sodium 1-ectane sulfonate） and detected at wave length of 200 nm and flow rate of 0.7 ml·min^-1. Results： Fairly good linearity for eampherol, isorhamnetin, meletin and betaine was obtained in the ranges of 0.2-4.8μg·ml^-1, 0.1-2.4μg·ml^-1, 0.025-0.8μg·ml^-1 and 1.0-32.0μg·ml^-1. The method precisions were 1.5%, 2.1%, 1.6% and 2.2%, respectively, and their amounts （%） in the Sanye Health Cigarette were 0.002 42, 0.000849, 0.000249 and 0.006 36, respectively. Condusion： The method is simple, convenient, rapid and accurate, and can be used for the determination of the major components in the Sanye Health Cigarette.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第2期210-213,共4页
Journal of Shandong University:Health Sciences
关键词
保健吸品
山奈酚
异鼠李素
槲皮素
甜菜碱
色谱法
高效液相
Health Cigarette
Campherol
Isorhamnetin
Meletin
Betaine
Chromatography, high performance liquid
