摘要
将pGFP-2质粒线性化，利用精子介导，通过人工授精技术导入意大利蜜蜂处女王，然后将授精王介绍到无王群繁殖后代。结果：试验群产生了一群绿色荧光阳性蜜蜂；对阳性蜂群后代分析发现，1-2日龄幼虫能检测到荧光，提取蜜蜂DNA进行PCR扩增得到预想的片断，通过RT-PC分析，从转录水平上证实转导的外源基因获得表达。结论：以绿色荧光蛋白（GFP）基因为报告基因，通过精子介导法能够生产克隆转基因蜜蜂。
The plasmid DNA was linearized and introduced with sperm during the instrumental insemination of virgin queen, then these inseminated queens were introduced into queenless colonies. Results ： a green fluorescence - positive colony was produced, where green fluorescence was observed for 1 to 2 - day - old lar- vae, the predicted fragment was isolated by means of PCR amplification of genomic DNA and the expression of transferred genes was confirmed at the transcriptional level by reverse transcription - polymerase chain reaction. Conclusion： using green fluorescent protein （GFP） as a reporter gene, transgenic honey bees could be established by SMGT.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2007年第1期118-123,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金资助项目(30300183)
关键词
意大利蜜蜂
精子
基因转移
GFP
Apis melliferia Ligustica
sperm
gene transfer
GFP
