摘要
用ISSR技术分析了38个桩果（Mangifera indica L．）品种的亲缘关系。从60条ISSR引物中筛选出10条重复性好、扩增带型清晰的引物，共扩增出79条可重复性带，多态性带比例为86．7％。扩增结果可区分38个品种中的37个（含红金龙或贵妃之一）。各品种间遗传距离在0～0．682之间，平均为0.393。UPGMA聚类将供试品种分成了5类。对分类结果进行了讨论。
The genetic relationships of 38 mango （Mangifera indica L. ） accessions were examined by ISSR markers. Of the 60 ISSR primers screened, only 10 primers gave reproducible, polymorphic DNA amplification patterns with a total 79 of amplified bands. Their percentage of polymorphic band was 86. 7%. By banding patterns obtained from these 10 primers, each accession could be distinguished from the others except either Hongjinlong or Guifei, since the two accessions had the same amplified band patterns. Genetic distances among the tested accessions ranged from 0 to 0. 682 with an average of 0. 393. Five groups were formed by UPGMA clustering and the classification results were discussed in this paper.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第1期87-92,共6页
Acta Horticulturae Sinica
基金
科技部科技基础性工作和社会公益研究专项面上项目(2003DIB7J066)
关键词
杧果
亲缘关系
ISSR
聚类分析
Mango
Mangifera indica L.
Genetic relationship
ISSR
Clustering analysis
