摘要
目的:研究高危型人乳头状瘤病毒感染及病毒载量与宫颈病变的关系。方法:对774例研究对象采用第二代杂交捕获试验(HC-Ⅱ)进行宫颈脱落细胞的HPV-DNA定量检测。分析不同程度宫颈病变的HPV感染情况,并根据HPV病毒载量将所有检测对象分为四组,阴性(RLU/CO<1.0)、低载量(1.0≤RLU/CO<10)、中载量(10≤RLU/CO<100)、高载量(RLU/CO≥100),采用非条件多项式logistic回归分析病毒载量与宫颈病变级别的关系。结果:在检测的774例研究对象中,对照组、宫颈上皮内瘤变(cervical intraepithelial neo-plasia,CIN)Ⅰ、CINⅡ、CINⅢ和浸润性宫颈癌患者的HPV感染率分别为21.29%、82.35%、80.00%、90.16%和86.67%。对照组高危型HPV感染率明显低于CIN和浸润性宫颈癌患者,差异有统计学意义,P<0.01;HPV阳性者患CIN的风险是阴性者的24.96倍(95%CI:13.20～47.18),患浸润性宫颈癌的风险是HPV阴性者的24.03倍(95%CI:12.01～48.09)。在不同级别宫颈病变中,CINⅠ组的低病毒载量患者占11.67%,高载量占41.18%,OR值为23.84(95%CI:5.96～95.33),P<0.001;而在CINⅢ组中62.30%的患者呈高病毒载量,OR值达64.70(95%CI:25.98～161.20),P<0.001。结论:高危型HPV感染与CIN和浸润性宫颈癌的发生密切相关;宫颈高危型HPV病毒载量是影响宫颈病变严重程度的危险因素。
OBJECTIVE： To explore the relationship between human papillomavirus （HPV） infection and cervical diseases. METHODS.. Seven hundred and seventy-four cervical smears were detected for high risk HPV-DNA by Hybrid Capture 2 systerm. The difference of HPV positive rates in subjects with various cervical diseases was analysed. All subjects were categoried into four groups according to the viral load.. negative （RLU/CO〈1. 0）, low viral load （1.0≤RLU/CO〈10）, medium viral load （10≤RLU/CO〈100）, and high viral load（RLU/CO≥100）. The relationship between viral load and various cervical diseases were analysed by unconditional multinomial logistic regression. RESULTS： The positive rates of HPV infection were 21.29% and 82.35^, 80.00%, 90.16%, 86.67% in control group and women with CIN Ⅰ , CIN Ⅱ, CIN Ⅲ, invasive cervical carcinoma, respectively. The positive rates were significantly higher in women with CIN and invasive cervical carcinoma （P〈0.01 ）. In HPV positive women, the risks for development of CIN and invasive cervical carcinoma were 24.96 （95% CI： 13.20 - 47.18） times and 24.03（95% CI：12.01-48.09） times higher than that in HPV negative women. In CIN Ⅰ group, there was 11.67% of patients in low viral load and 41.18% in high viral load, the odds ratio was 23. 84（95% CI： 5.96-95.33）（P〈0.001）. In CIN Ⅲ group, the proportion of high viral load accounted for 62.30% with odds ratio 64.70 （95% CI： 25.98- 161.20） （P〈0. 001）. CONCLUSIONS： There is a close relationship between HPV infection and the development of CIN and invasive cervical carcinoma. The viral load of high risk HPV is a risk factor that could influence the degree of cervical diseases.
出处
《中华肿瘤防治杂志》
CAS
2007年第1期5-8,共4页
Chinese Journal of Cancer Prevention and Treatment
