摘要
目的探讨两肾一夹(2K1C)高血压大鼠肾脏血管紧张素转换酶2(ACE2)表达。了解甘肃黄芪对大鼠肾脏ACE2mRNA表达及血浆血管紧张素Ⅱ(AngⅡ)的影响,观察黄芪的降压效果。方法50只雄性Wistar大鼠随机分为5组:正常对照组(n=10);2K1C高血压组(n=10);2K1C+缬沙坦组(n=10);2K1C+黄芪20g/(kg.d)组(n=10);2K1C+黄芪10g/(kg.d)组(n=10)。8周后,用RT-PCR法检测肾脏ACE2mRNA表达,免疫组化法检测肾脏ACE2表达,放射免疫分析法测定肾脏局部AngⅡ水平。结果2K1C高血压大鼠肾脏ACE2mRNA表达较正常大鼠降低(0.282±0.025vs0.359±0.017,P<0.05),高、低剂量黄芪及缬沙坦治疗大鼠ACE2mRNA表达(0.398±0.004,0.391±0.006,0.403±0.009)均高于对照组(0.282±0.025)(P<0.05)。高剂量黄芪组降低肾脏局部AngⅡ效果较缬沙坦组明显(P<0.01)。缬沙坦和黄芪治疗都明显降低血压(P<0.01)。结论2K1C高血压大鼠肾脏组织中ACE2mRNA表达降低。甘肃黄芪及缬沙坦在降压的同时增加肾脏组织ACE2mRNA表达。
Objective To determine the expressions of angiotensin-converting enzyme 2（ACE2） in two-kidney and one-clip（2K1C） hypertensive rats and the effect of Radix Astragali. Methods Fifty adult male Wistar rats were groupped as follows： control group（n=10）, 2K1C renal hypertension group（n=10）, 2K1C＋high dose Radix Astragali group [20 g/（kg · d）, n=10]; 2K1C＋low dose Radix Astragali group（10 g/kg · d, n=10）, 2KIC＋ valsartan group （ 20 g/kg · d, n= 10） for 8 weeks. The levels of mRNA expression of ACE2 was determined by RT-PCR and the distribution and expression of ACE2 in kidney by immunohistochemistry. Renal Ang Ⅱ was measured with radioimmunoassay. SBP were measured by tail-cuff method. Results ACE2 mRNA in kidney were found to be significantly decreased in the 2K1C hypertensive rats compared with those of the control group （0. 282±0. 025 vs 0. 359±0. 017, P〈0.05）. Radix Astragali and valsartan treatment increased ACE2 mRNA （0. 398±0. 004, 0. 391±0. 006 vs control： 0. 403±0. 009, P〈0. 05）. Both Radix Astragali and valsartan decreased BP significantly（P〈0.01）, however high dose Radix Astragali decreased renal Ang Ⅱ more markedly than those by valsartan （ P〈0.01 ）. Conclusion The renal ACE2 is down-regulated and the level of Ang Ⅱ is increased in 2K1C group. RadixAstragali and valsartan increase ACE2 in renal tissue along with BP decreases.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2007年第1期39-43,共5页
Chinese Journal of Hypertension
基金
甘肃省科学事业费项目(QS041-C33-17)
甘肃省自然科学基金项目(ZS031-A25-054-E)
关键词
高血压
血管紧张素转换酶2
基因表达
缬沙坦
黄芪
Hypertension
Angiotensin-converting enzyme 2
Gene expression
Valsartan
Radix Astragali
