摘要
本研究建立了反相高效液相法测定新药糖渴清片中小檗碱、掌叶防己碱和药根碱含量的方法。固定相为ＹＷＧＣ１８．１０μｍ，４．６（ｉ．ｄ．）×２５０ｍｍ柱；流动相为乙腈－水（１：１，每１０００ｍｌ含磷酸二氢钾３．４ｇ及十二烷基硫酸钠１．７ｇ）；检测波长为３４５ｎｍ；小檗碱、掌叶防己碱和药根碱的加样回收率分别为９６．００±１．９９％，９８．９２±１．６２％，９７．２８±１．６２％。
A reversed-phase HPLC method was described for the determination of berberine, palmatine and jatrorrhizine in Tangkeqing Tablet. The three contituents were separated on a YWG C18 4. 6(i. d) × 250mm column,using acetonitrile-water, 1: 1, and each 1000ml contains 3. 4g KH2PO4 and 1. 7g CH3(CH2) 11SO4Na, as the mobile phase, detected at 345urn. The average recoveries of the three contituents were 96. 00 ± 1. 99%, 98. 92±1. 62% and 97.28 ± 1. 62%.
出处
《南京中医药大学学报》
CAS
CSCD
1996年第6期25-27,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
省教委自然科学基金
关键词
糖渴清片
小檗碱
掌叶防己碱
药根碱
reversed-phase HPLC method
Tangkeqing Tablet
berberine
palmatine
jatrorrhizine
