摘要
为研究连翘叶黄酮(Forsythia suspenseleaves flavonoids,FLF)的体外抗氧化作用,用水杨酸法研究FLF清除.OH的效果,并测定了FLF对连苯三酚自氧化体系的抑制作用。用硫代巴比妥酸(TBA)法测定了小鼠4种器官及肝线粒体、微粒体中的丙二醛(MDA)含量,用分光光度法测定了小鼠红细胞溶血和肝线粒体膨胀程度。结果表明,FLF可以清除.OH,抑制连苯三酚自氧化,并抑制.OH所致丙二醛的产生,减少红细胞溶血,减轻肝线粒体膨胀程度。说明FLF具有明显的抗氧化活性。
To study the in vitro antioxidant activity of the Forsythia suspense leaves flavonoids （ FLF）, the scavenging effect of FLF on hydroxyl radicals（ · OH ） was studied by salicylic acid method,and self-oxidation inhibition was studied using the pyrogallol. The contents of MDA in 4 organs, and in mitechondria and mierosome of liver were measured by TBA assay. The extent of erythrocatalysis and the swelling extent of mitochondria of liver were detected by spectrophotometric methods. The result showed that FLF had the · OH and O2^-· scavenging activity. It could inhibit the generation of MDA,the swelling of mitechondria,and the hemolysis of red blood cells. The conclusion was drawn that FLF have the antioxidant activity.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第1期97-100,共4页
Natural Product Research and Development
基金
陕西省科技攻关项目(2004K18-G10)
关键词
连翘叶黄酮
抗氧化
体外
自由基
Forsythia suspense leaves flavonoids（FLF）
anti-oxidation
in vitro
free radical
