摘要
目的建立反相高效液相色谱法同时测定血清中五种苯二氮[艹卓]类药物浓度。方法采用Hypersil C18（4．6mm×250mm，10μm）色谱柱，甲醇-乙腈-水（48：14：38，V／V）为流动相，检测波长为254nm，室温检测。结果五种苯二氮蕈类药物分离较好，浓度在0．15～40μg·mL^-1范围内线性关系良好，回收率为96．3％～104．4％，日内及日间精密度RSD〈4．7％。结论本法简便、快速，适用于临床血药浓度的常规监测和中毒病人的急救诊断。
OBJECTIVE A HPLC method was established for determination of five Benzodiazepines in serum concentration. METHODS Hypersil C18 （4. 6mm × 250mm, 10μm）colum was used. the mobile phase was composed of Methanval-acetonitrile-water （48 ： 14 ： 38, V/V）, and the detecting wave-length was 254nm. RESULTS The linearity of Ben-zodiazepines in serum was in the range of 0.15μg · mL^-1 - 40μg · mL^-1. The recovery was 96.3 % -104. 4 % ,RSD of with-day and between-day were less than 4. 7 %. CONCLUSION This method is simple,quick and accurate for determination of five Benzodiazepines concentration in serum.
出处
《海峡药学》
2007年第3期50-52,共3页
Strait Pharmaceutical Journal
