摘要
目的：建立测定人血浆中泛昔洛韦人体内活性代谢物喷昔洛韦的高效液相色谱法。方法：血浆样品用10％三氯醋沉淀蛋白。色谱柱为Zorbax SB-C18柱（150mm×4．6mm，5μm），流动相为0．02mol·L^-1磷酸二氢钾溶液（pH7．0）-甲醇-乙腈（90：10：0．1），流速为1．0mL·min^-1，紫外检测波长为254nm。结果：血浆中内源性物质对样品测定无干扰。本方法线性范围为0．05～5mg·L^-1（r=0．9994），最低定量质量浓度为0．05mg·L^-1，方法回收率为100．1％～101．5％，日内、日间RSD均小于7％。结论：本法简便、灵敏、准确，适用于泛昔洛韦药动学的研究。
OBJECTIVE To establish an HPLC method for the determination of penciclovir, the active metabolite found in human plasma after administration of famciclovir. METHODS Proteins in plasma samples were precipitated by trichloroacetic acid. Penciclovir was separated on a Zorbax SB-C18 column（ 150 mm× 4. 6 mm, 5 μm） with the mobile phase consisted of 0. 02 mol·L^- 1 potassium dihydrogen phosphate solution（pH 7. 0）-methanol-acetonitrile （90： 10：0. 1 ） at a flow rate of 1.0 mL· min^- 1. The UV detection wavelength was set at 254 nm. RESULTS The blank plasma did not interfere with the determination of the analytes. The linear range was in the range of 0. 05 - 5 mg·L^-1 （r= 0. 999 4）. The limit of quantitation was 0. 05 mg· L^- 1. The method recovery was 100.1%- 101.5 %. Within-day and between-day RSD were less than 7 %. CONCLUSION The method is simple,sensitive and accurate,which is suitable for the pharmacokinetic study of famciclovir.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第3期346-348,共3页
Chinese Journal of Hospital Pharmacy
