摘要
运用逐点扫描(Scanning)采谱技术,测量了人体乳腺肿瘤组织的阵列拉曼光谱集(Mapping);利用自编的软件,分析和计算了该谱集与参考谱DNA、细胞质等拉曼谱的相关系数;并将其映射为灰度值,构建了点阵的相关系数成像。结果表明,相关系数成像较好地反映了参考谱所代表的生化成分的分布;DNA谱与组织中其他成分谱的相关性很小,因此DNA谱的相关系数成像能够直接地反映成像区域DNA的分布,可信度尤其高。该研究结果为进一步研究乳腺肿瘤的拉曼诊断方法奠定了一定基础。
The Raman mapping spectra were collected from the samples of fresh human breast tumor tissues with the excitation wavelength at 633 nm, their correlation coefficients with the reference spectra of chemical DNA, cytoplasm and extra-cellular matrix etc. were calculated, and the images were created based on these correlation coefficients. Examining these images, the authors found that they show approximately the distribution of the corresponding reference chemicals in human tissues and that the DNA spectrum correlation images reflect the DNA distribution quite well because of its much lower correlation with the spectra of other morphological chemicals. This work is helpful in understanding the chemical/morphological basis of the breast tissue Raman spectra and in developing the Raman diagnostic method for breast tumor.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第2期295-298,共4页
Spectroscopy and Spectral Analysis
基金
北京市教委科技发展计划项目(KM200710017008)资助
关键词
乳腺肿瘤
拉曼光谱
点扫描成像
相关系数
成像
Breast tumor
Raman spectrum
Mapping
Correlation coefficients
Imaging
