摘要
头孢呋辛钠、头孢曲松钠、头孢噻肟钠和头孢唑啉钠在紫外区均有吸收，且吸收光谱重叠而相互干扰，难以直接进行分析。在pH2．09的Britton-Robinson（B-R）缓冲溶液中采用紫外分光光度法对其混合溶液进行测定，所得的波谱数据用化学计量学多元校正方法进行了处理。四种β-内酰胺类抗生素的的线性范围分别为1．0～20．0μg·mL^-1，2．0～20．0μg·mL^-1，2．0～20．0μg·mL^-1和1．0～18．0μg·mL^-1。利用此方法还测定了兔血清中的四种伊内酰胺类抗生素，与高效液相色谱法所获得的结果进行比较，两者无显著性差异。
Cefuroxime sodium, ceftriaxone sodium, cefotaxime sodium and cefazolin sodium have absorption in ultraviolet region, and their absorption spectra are overlapping. So they can not be determined individually by spectrophotometry without prior separation. In this paper, the chemometric multivariate calibration method was applied to the simultaneous determination of these four compounds in a Britton-Robinson buffer solution （pH 2.09）, and the analytical results were compared with those by classical least squares （CLS）, principal components regression （PCR） and partial least squares （PLS）. The linear ranges of cefuroxime sodium, ceftriaxone sodium, cefotaxime sodium and cefazolin sodium are 1.0-20.0, 2.0-20. 0, 2.0-20.0 and 1.0-18. 0 μg · mL^-1 , respectively. The proposed procedure was successfully applied in the determination of these drugs in rabbit serum, and the result obtained from spectrophotometry was compared with the one by HPLC with no significant difference found.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第2期355-359,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20365002
20562009)
江西省自然科学基金项目(0620041)
长江学者创新团队项目(IRT0540)资助
