摘要
目的:探讨血管紧张素Ⅱ受体1和受体2在小鼠肾脏发育早期输尿管芽的表达及其与肾脏发育的关系。方法:实验于2005-05/2006-05在锦州医学院组织与胚胎学教研室完成。①取成年健康昆明系小白鼠24只,体质量25～30g,雌、雄(1∶1)同窝饲养,见雌鼠阴道栓出现计为胚龄0d。取胚龄12,14,15,16日胎鼠,各龄小鼠每组4只。剖腹取出肾脏,40g/L甲醛固定,制成切片5μm。②常规苏木精-伊红染色光镜下观察小鼠肾组织发育早期形态学变化。③采用免疫组织化学方法检测胚龄12,14,15,16d小鼠肾脏发育早期输尿管芽分支中血管紧张素Ⅱ受体1和受体2的含量。④体视学测量:每个动物的肾脏系统随机选取5张切片,按“S”形等间隔选取视野,采用方格测试系统,交点计数法测算血管紧张素Ⅱ受体1和受体2在输尿管芽阳性表达的体积密度。结果:①小鼠肾组织发育早期形态学观察:胚龄12d小鼠肾脏中可见输尿管芽及其分支。胚龄15d,输尿管芽周围出现了逗号小体、S小体,但未见成熟肾小体。胚龄16d的肾脏中可见出现了成熟肾小体、近曲小管和远曲小管。②小鼠肾脏组织免疫组织化学染色结果:在胚龄12d,血管紧张素Ⅱ受体1和受体2在输尿管芽中均有少量表达,胚龄15d,血管紧张素Ⅱ受体1达到高峰,胚龄16d血管紧张素Ⅱ受体1仅有微量表达;胚龄14d,血管紧张素Ⅱ受体2达到高峰,以后逐渐降低。结论:输尿管芽在小鼠后肾发生中有血管紧张素Ⅱ受体1和血管紧张素Ⅱ受体2表达,且血管紧张素Ⅱ受体1与诱导输尿管芽分支不断延长有关。
AIM： To observe the expression of angiotensin Ⅱ AT1 and AT2 receptor in uretenc bud cell Ot mouse and investigate the relationships between it and kidney development.
METHODS： The experiment was performed in the Department of Histology and Embryology, Jiozhou Medical College from May 2005 to May 2006.①A total of 24 Kunming adult mice of either gender were bred together, weighed 25-30 g. The time that pessary fell off was termed as embryonic 0 day. The mice at embryonic days 12, 14, 15, 16 were selected, with 4 mice in each. The kidneys were taken, fixed with 40 g/L formaldehyde and were cut into 5-μg-thick sections. ②Morphometric changes were examined by hematoxylin-eosin dyeing under light microscope during the mouse kidney development.③The expression of AT1 and AT2 receptor was examined in ureteric bud cell of the embryonic mouse, including embryonic days 12, 14, 15, 16 by immunohistochemical technique.④Measurement of stereology： Five fields of view were selected randomly in each section according to ＂S＂ shape, The square test system＂ and point of intersection method were used to calculate the volume density of angiotensin Ⅱ AT1 and AT2 receptor in ureteric bud cell.
RESULTS： ①Morphometric observation： At E12, ureteric bud cell branches emerged. At E15, comma-shaped bodies and S-shape bodied emerged. At E16, mature renal corpuscle, proximal＇convoluted tubule and distal convoluted tubule emerged.②Immunohistochemical dyeing： At E12, the expressions of AT1 and AT2 receptor were observed at low levels in ureteric bud cell branching. At E15, the expression of AT1 receptor peaked. At E16, the expression of AT1 receptor was present little. At E14, the expression of AT2 receptor was observed highly abundant and then decreased gradually.
CONCLUSION： Ureteric bud cell branching expresses AT1 and AT2 receptor dudng murinemetanephrogenesis. AT1 receptor expression appears to correlate with the branching of ureteric bud cell.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第10期1858-1860,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
