摘要
以甘蓝型油菜带柄子叶为转化受体,通过根癌农杆菌EHA105(Agrobacterium tumefaciens)介导将盐角草(Salicornia)的Na+/H+反向运输体基因NHX导入新疆主栽油菜1khp11品系,获得了抗卡那霉素的再生植株,并对影响遗传转化的一些关键因素进行了研究。实验结果表明:带柄子叶在含有2,4-D的培养基上经过2d短时间的预培养后在菌液浓度OD600为0.3时在28℃摇床浸染15min时卡那抗性绿苗率可达10%￣12%;经过抗性植株的PCR及RT-PCR检测证明外源基因已整合到油菜基因组中,并通过表型实验证实由于外源基因的导入提高了植株的耐盐能力。
Cotyledons with petiole of Xinjiang Brassica napus variety 1khp11 variety were transformed with Agrobacterium tumefaclens EHA105,in which is harboring Na^＋/H^＋ transport gene NHX of Salicornia, and the kanresistant were regenerated a native halophyte in xinjiang, and andysed the key factors affecting the genetic transformation, The results showed that kan-resistant plantlets can be obtained and NHX gene has already inserted into genome of oilseed by PCR and RT-PCR, The salt tolerance ability of transgertic oilseed was significant improved by introduction of NHX gene of Salicornia.
出处
《生物技术通报》
CAS
CSCD
2006年第3期81-84,共4页
Biotechnology Bulletin
基金
新疆高校创新研究群体基金项目(XJEDU2004G02)
教育部科学技术研究重点项目(205178)
关键词
耐盐基因NHX
新疆盐生植物盐角草
油菜带柄子叶
耐盐能力
Salt tolerant gene NHX Halophyte salicornia in xinjiang Cotyledons with petiole of brassica napus Salt tolerance
