摘要
肿瘤坏死因子（ＴＮＦ）除抗肿瘤活性外，还具有其它广泛的生物学活性，但因其严重的毒副作用极大地限制了它的临床应用。在ＴＮＰ结构与功能关系研究的基础上，该文作者利用ＰＣＲ技术构建了一种新型人ＴＮＦ的编码基因，并使其在大肠杆菌中进行了高效表达。活性测定结果表明，新型ＴＮＦ对Ｌ９２９细胞的细胞毒活性较天然型人ＴＮＰ增高了约７１４倍。
Alhough TNF-a has widespread biological activities in addition to its antitumor activity ,its severe toxicity limits its use in clinic. We constructed a new type gene encoding human TNP-o by using polymerase chain reaction CPCR technique. The gene was highly expressed in E. coli. Activity assay indicated that the cytotoxicity of the new type TNF on L929 cell line was 714 times higher than that of wild type human TNF.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第4期254-258,共5页
Chinese Journal of Microbiology and Immunology
关键词
肿瘤坏死因子
突变体
PCR
高活性
Tumor necrosis factor
Mutant Ploymerase
chain reaction
