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一株运动发酵单胞菌Zy-1快速生产乙醇 被引量:12

Fast Production of Ethanol by Zymomonas mobilis (Zy-1)
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摘要 经多次实验优化,得到运动发酵单胞菌Zy发酵葡萄糖生产乙醇较合适的条件.Zy的诱变菌株Zy-1在该条件下发酵葡萄糖生产乙醇比原始菌株更有较大优势.当葡萄糖浓度为200gL^-1时,发酵48h,乙醇浓度96.5gL^-1,残糖2.3gL^-1,发酵效率为94.42%.Zy-1发酵天然原料米粉、木薯、红薯干等,发酵时间44h,乙醇浓度达95gL^-1以上,发酵效率92%以上.发酵液用DNS法测定,还原糖约2gL^-1,残总糖因原料种类不同,其值有所差异(5~20gL^-1).经薄层层析分析,发酵液无葡萄糖,而是二糖、三糖等低聚糖. A good condition for fermenting glucose into, ethanol by Zymomonas mobilis (Zy) was achieved. Under the condition, its mutant ( Zy-1 ) was more capable of producing ethanol than its original strain. When glucose concentration was 200 g L^-1, the ethanol concentration was 96.5 g L^-1 and stoichiometric yield was 94.42% by Zy-1 fermentation for 48 h. In addition, as Zy-1 fermented raw materials, such as rice flour, cassava flour and sweet potato into ethanol under the optimum condition, the ethanol concentration could be over 95 g L^-1 and stoichiometric yield above 92%, with fermentation time of 44 h. The sugar concentration in fermentation liquor was measured by the DNS method. The reducing sugar was about 2 g L^-1 , but residual sugars were different owing to different raw materials (from 5 g L^-1 to 20 g L^-1 ). The analysis by thin-layer chromatogram indicated that in the fermentation liquor there was no glucose, but there were disaccharide and trisaccharide. Fig 2, Tab 5, Ref 12.
出处 《应用与环境生物学报》 CAS CSCD 北大核心 2007年第1期69-72,共4页 Chinese Journal of Applied and Environmental Biology
基金 科技部攻关项目(2004BA411130) 中国科学院"西部之光"项目 四川省科技攻关项目"细菌发酵燃料乙醇制备技术研究"资助(04NG002-002 027.X028-037)~~
关键词 运动发酵单胞菌(Zymomonas mobilis) 细菌发酵 乙醇 诱变菌株 米粉 木薯 红薯干 Zymomonas mobilis bacterial fermentation ethanol mutant rice flour cassava flour sweet potato CLC TQ920.1
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参考文献11

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二级参考文献24

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