摘要
[目的]建立一种快速、简便、灵敏的测定血清总蛋白的方法。[方法]在非离子型表面活性剂聚氧乙烯月桂醚(Brij-35)存在下,用水溶性试剂偶氮胂Ⅲ(ArsenazoⅢ)作显色剂直接分光光度法测定血清总蛋白。[结果]该方法测定蛋白质,显色络合物的最大吸收波长为610nm,线性范围在0～56.2mg/L,表观摩尔吸光系数为3.60×10~5L.mol^(-1).cm^(-1)。回收率为96.1%～103.6%,批内相对标准差和批间相对标准差(RSD)分别为0.8%与2.0%,与双缩脲法比较相关性良好,Y=0.9882X+1.1007,r=0.9898,P﹥0.05。59名健康成年人血清总蛋白含量为(62.9～80.8)g/L(±2s)。[结论]该法血清用量少,线性范围宽,具有操作快速、简便、结果灵敏可靠等优点,适用于血清总蛋白的手工测定和自动分析。
[Objective] To establish a simple, sensitive and stable method of determining of total protein in serum by spectrophotemetry. [ Methods] Arsenazo Ⅲ was used as a coloring reagent to determine protein by spectrophotometry in the presence of surfactants Brij-35. [Results] The maximum absorption of the compound was at 610 van. The linearity of the test was 0-56.2 mg/L. The molar absorption coefficient was 3.60×10^5L·mol^-1·cm^-1. The recoveries ranged from 96.1% to 103.6%. The within-run and between-run CVs were 0.8%-1.5%and 1.5%-2.0%.Comparing this method （Y） with Biuret method, we obtained the regression equation Y = 0.9882X＋1.1007, r = 0.9898, P 〉 0.05, the reference interval （x^-±2s） was （62.9- 80.8） g/L （n = 59） . [Conclusion] This method is simple, sensitive, rapid and accurate, and it＇s suitable for the routine manual or automatic analysis of total protein in serum for clinical application.
出处
《现代预防医学》
CAS
北大核心
2007年第4期810-811,共2页
Modern Preventive Medicine
