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金纳米微粒辅助细胞激光热作用疗法研究 被引量：4

Laser Irradiation Cell Photothermal Therapy Assisted by Gold Nanoparticles
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摘要 金纳米微粒对可见光的强吸收特性使得光能可以高效地转换为热能.由于金纳米微粒的尺度在几十纳米范围,并且很容易与其他生物体结合,因此可以在局部范围进行激光选择性加热,这非常适合作为分子或细胞的靶向.采用这种金纳米微粒辅助激光热作用方法,对牛肠碱性磷酸酯酶(alkaline phosphatase aP)的选择性破坏,细胞膜的通透性提高以及对细胞的选择性灭活进行了试验并得到了很好的结果.此外,还讨论了用这种方法进行基因转染以及选择性光热治疗一些疾病的可能性. The strong absorption of gold nanoparticles in the visible spectral range allows the localized generation of heat in a volume of only a few tens of nanometer. The efficient conversion of strongly absorbed light by plasmonic gold nanoparticles to heat energy and their easy bioconjugation suggest that the gold nanoparticles can be used as selective photothermal agents in molecular cell targeting. The selective destruction of alkaline phosphatase, the permeabilization of the cell membrane and the selective killing of cells by laser irradiating gold nanoparticles were demonstrated. The potential of using this selective technique in molecularly targeted photothermal therapy and transfection is discussed.
作者 姚翠萍 张镇西 姚保利
机构地区 中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室 西安交通大学生物医学信息工程教育部重点实验室生命科学与技术学院生物医学工程研究所
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2007年第3期312-316,共5页 Progress In Biochemistry and Biophysics
基金 国家自然科学基金资助项目(60578026) 瞬态光学与光子技术国家重点实验室开放基金资助项目~~
关键词 激光照射 金纳米微粒 生物分子 细胞 laser irradiation, gold nanoparticles, biomolecules, cell
分类号 R318.15 [医药卫生—生物医学工程]
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参考文献16

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二级参考文献14

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共引文献6

同被引文献128

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引证文献4

二级引证文献10

生物化学与生物物理进展
2007年 第3期

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