摘要
目的:观察扶正养营颗粒对免疫介导再生障碍性贫血小鼠骨髓增殖及bcl-2表达的影响。方法:BALB/c小鼠40只随机分为正常组、模型对照组、环孢霉素A对照组、扶正养营颗粒实验组,记数外周血象、骨髓有核细胞,测定骨髓造血组织容量及bcl-2表达。结果:与正常组比较,模型组小鼠外周血象记数、骨髓有核细胞数、造血组织容量及bcl-2表达显著下降,差异有统计学意义(P<0.05);与模型组比较,环孢霉素A组、扶正养营颗粒组外周血象记数、骨髓有核细胞数、造血组织容量及bcl-2表达显著增加,差异有统计学意义(P<0.05)。结论:扶正养营颗粒可能是通过上调拮抗凋亡因子bcl-2的表达,改善骨髓增殖,发挥其治疗再生障碍性贫血的作用。
Objective To explore the effect of Fuzhengyangying granules （FZYYG） on the expression of the bone marrow proliferation and bcl-2 in mice with immune meditated aplastic anemia. Methods Forty BALB/c mice were randomly allocated to 4 groups ： a normal control group, a model group, cyclosporine A （CSA） group and FZYYG group. The model group was fed with physical salt, while the CSA and FZYYG group were fed with CSA and FZYYG respectively, and then the changes of peripheral hemoglobin （ Hb ） , platelet, bone marrow nucleus cell （ BMNC ） , bone marrow hematopoiesis tissue volume and bcl-2 expression were examined. Results The count of Hb, the platelet, BMNC, the bone marrow hematopoiesis tissue volume and the bcl-2 positive rate in the model group were lower than those in the normal group （ P 〈 0.05 ） , whereas the count of Hb, the platelet, BMNC, the bone marrow hematopoiesis tissue volume and the bcl-2 positive rate in both CAS and FZYYG groups were significantly higher than those in the model group （ P 〈 0.05 ）. Conclnsion FZYYG can induce the bcl-2 antigen expression and postpone the haematogenous cells apoptosis, and it is effective for mice with immune meditated aplastic anemia.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期88-92,共5页
Journal of Central South University ：Medical Science
基金
湖南省卫生厅基金资助(202104)~~
