摘要
目的建立克痤隐酮乳膏中隐丹参酮的含量测定方法。方法高效液相色谱法，迪马C18（250mm×4．6mm，5．0μm）色谱柱，甲醇-水（150：55）为流动相，检测波长为263nm。结果隐丹参酮在0．1152～0．2688μg范围内进样量与峰面积线性关系良好，平均回收率为96．6％，RSD为0．59％，准确率高。结论本方法简便、准确，可准确测定克痤隐酮乳膏中隐丹参酮的含量。
Objective To establish an assay of Cryptotanshinone in Kezuo Yimong Rugao by HPLC.Methods HPLC column was Diamonsil C18（250mm×4.6mm,5.0μm）, mobile phase was methanol - water（150：55）, detection wavelength was 263 nm. Results The calibration curve was linear in the range of 0.1152 μg - 0.2688 μg for berberine hydrochlorrde. The average recovery was 96.6 % and RSD was 0.59%. Conclusion The method was simple,rapid,accurate and reliable.
出处
《黑龙江医学》
2006年第9期666-667,共2页
Heilongjiang Medical Journal
