摘要
目的观察罗哌卡因腹腔注射后对大鼠血液淋巴细胞DNA的影响,为不同浓度罗哌卡因的应用提供安全性依据。方法Wistar大鼠72只,随机分为四组,对照组腹腔注射生理盐水,实验组(T1,T2,T3)按体重0.1 mL/100 g分别腹腔注射0.5%、0.75%、1.0%罗哌卡因。各组分别在腹腔注射后6、12和24小时3个时点各取6只动物,采用单细胞凝胶电泳检测血液淋巴细胞DNA损伤。结果各实验组血淋巴细胞的彗星率和尾长均高于对照组,其中T3组各时点与对照组相比差异有统计学意义(P<0.05);T2组与对照组相比,在24小时时差异有显著性(P<0.05)。结论低浓度(0.5%,0.75%)罗哌卡因腹腔注射24小时内对大鼠血液淋巴细胞无明显影响;高浓度罗哌卡因(1.0%)腹腔注射24小时内对大鼠血液淋巴细胞可能存在损伤作用。
Objective To explore the lymphocyte DNA damages after celiac injection ropivacaine in wistar rats and provide the experimental data for the security assessment of ropivacaine application. Methods Seventy - two male wistar rats were randomly and averagely divided into 4 groups. The groups were randomized to receive normal saline, 0.5%, 0.75% or 1.0% respectively by the celiac injection 0.1 mL every 100 g body weight. Six rats in every group in 6 h, 12 h and 24 h after the injection were killed, and every rat was phlebotomized blood approximately 0.5 mL blood{anticoagulant by heparitin）. The alkaline single cell gel electrophoresis was used to determine DNA damages of blood lymphocyte. Results The comet tail ratio and tail length of lymphocyte in 1.0% concentration group at 6 h, 12 h and 24 h were higher or longer than that of the control group at corresponding time point （ P 〈 0.05）, while that of 0.75 % ropivavaine group compared with the control was significantly different only in 24 h（ P 〈 0.05）. The comet tail ratio and tail length of lymphocyte in 0.5% concentration group at 6 h, 12 h and 24 h compared with the control had no significant difference （ P 〉 0.05）. Conclusions 0.5 % and 0.75 % ropivavaine do not have obvious DNA damage effect on rats＇ lymphocyte. But 1% ropivavaine can induce DNA damages of rats lymphocyte, and it may have potential risk to human.
出处
《南华大学学报（医学版）》
2007年第1期71-73,共3页
Journal of Nanhua University(Medical Edition)
