摘要
目的探讨肺动脉和主动脉炎症发生时,血管生成的作用机制。方法采用定量PCR及Western blot方法检测肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)刺激原代人主动脉内皮细胞(HAECs)和(或)原代人肺动脉内皮细胞(HPAECs)后,Tie 2基因的表达情况。结果TNF-α和IL-1β均能增加原代血管内皮细胞Tie2基因的表达。TNF-α作用于HAECs,HPAECs及IL-1β作用于HPAECs 24h后,Tie 2的表达明显增加;在HPAECs,两者的作用均在36h达高峰。结论炎症刺激因子TNF-α和IL-1β在人血管内皮细胞可诱导Tie 2基因的表达,它可能与炎症状态下的血管形成有关。
Objective To investigate the expression of Tie 2 gene induced by inflammation cytokines TNF-α and IL-1β in vascular endothelium cells and explore the mechanism of angiogenesis under inflammation. Methods mRNA and protein expressions of Tie 2 were investigated by quantitative PCR and Western blot in primary human aortic endothelial cells （HAECS）and/or primary human pulmonary artery endothelial cells （HPAECS）induced by inflammatory cytokines, Results TNF-α and IL-1β could increased the expression of Tie 2 in primary endothelial cells. The expression of Tie 2 was significantly increased 24 hours after stimulation of TNF-α in HAECS and HPAECS and IL-1β in HPAECS. in HPAECS, the effect of both arrived the peak at 36 hours after stimulation. Conclusion The expression of Tie 2 could be increased by inflammatory cytokines TNF-α and IL-1β in human vascular endothelial cells. It possibly contributes for angiogenesis under inflammation.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2007年第2期65-67,共3页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
留学回国人员科研启动基金(2005)
广东省自然科学基金资助(5100992)
