摘要
本试验在最佳诱导条件下获得猪圆环病毒Ⅱ型重组Cap蛋白的基础上,利用His Bind蛋白质纯化试剂盒对表达产物进行纯化,通过Western blot检测证明表达产物具有良好的抗原性。以纯化后的重组融合蛋白作为诊断抗原,对ELISA反应条件进行优化,初步建立了检测PCV2抗体的间接ELISA方法。用该法对广东、广西一些地区收集到的380份血清样品进行检测,检测结果阳性率为86.1%,从中随机取90份猪血清样品与国产商品化试剂盒检测结果对比,符合率为95.6%,表明本实验建立的间接ELISA方法具有较高的敏感性和特异性,适于大规模检测PCV2血清抗体的流行病学调查。
An indirect enzyme-linked immunosorbent assay （ELISA） was developed based on a recombinant capsid protein of porcine circovirus type 2 （PCV2）. The recombinant capsid protein was expressed under the optimum induced condition, purified by a His Bind Purification Kit, and confirmed by Western blot assay. The indirect ELISA was used to test against 380 serum samples collected from Guangdong and Guangxi province and 86.1% samples were positive. Compared with commercial ELISA kits, the assay showed a correlation rate of 95.6 % based on the data obtained from 90 randomly picked serum samples. The results indicated that the indirect ELISA is not only sensitive and specific, but also suitable for large-scale epidemiological investigation for PCV2 infection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2007年第3期217-221,共5页
Chinese Journal of Preventive Veterinary Medicine
关键词
PCV2
重组CAP蛋白
表达
纯化
ELISA
porcine circovirus type 2
recombinant capsid protein
expression
purification
ELISA
