摘要
目的探讨地塞米松（dexamethasone，DXM）对脂多糖（1ipopolysaccharide，LPS）诱导的人外周血单核细胞核因子（nuclearfactorkappaB，NF-κB）活化和肿瘤坏死因子（tumornecrosisfactor-α，TNF-α）产生的影响。方法收集人外周血单核细胞接种于培养板后，分生理盐水对照组、LPS组、LPS＋DXM1μmol／L组、LPS＋DXM10μmol／L组。LPS诱导0、0．5、2、6、12、16h后留取细胞及细胞培养上清。采用免疫细胞化学染色法检测细胞NF．船p65阳性表达率，酶联免疫吸附法检测细胞培养上清中TNF-α的含量。结果LPS诱导0．5h后NF-κB表达开始增加，2h达高峰。2h时TNF-α合成开始增加，6h达高峰。在2、6、12h时，LPS＋DXM1μmol／L组和10μmol／L组TNF-α含量均比LPS组各相应时间点低（P〈0．05或0．01）。在0．5、2、6h时LPS＋DXM1μmol／L组NF-κB阳性表达率和在2、6h时LPS＋DXM10μmol／L组NF-κB阳性表达率均比LPS组低（P〈0．05或0．01）。LPS＋DXM1μmol／L和10μmol／L组NF-κB阳性表达率和TNF-α含量在各时间点差异均无统计学意义。结论LPS在体外诱导人外周血单核细胞NF-κB的活化和TNF-α的产生；DXM通过抑制NF-κB的活化和TNF-α的释放而发挥抗炎作用，并且这种抑制效应可能不随剂量的增加而增强。
Objective To investigate the effects of dexamethasone （DXM） on activation of nuclear factor kappaB （NF-κB） and production of tumor necrosis factor-α （TNF-α） in lipopolysaccharide （LPS） -induced human peripheral blood monocytes. Method The cultured human peripheral blood monocytes were divided into 4 groups： normal saline control group, LPS treated group, LPS plus DXM 1 μmol/L treated group, LPS plus DXM 10 μmol/L treated group. At 0, 0.5, 2, 6, 12 and 16 hours after stimulation, the cells and culture supernatants were obtained. The content of TNF-α in cultured supematant was measured by ELISA. The positive rate of NF-κB p65 expression in monoeytes was determined by immunocytoehemistry. Results The expression of NF-κB of cultured monocyte began to increase after LPS-induced for 0.5 hours and reached the peak at 2 hours. The production of TNF-α of culture supematants began to increase after LPS-induced for 2 hours and reached peak at 6 hours. DXM significantly suppressed LPS-induced TNF-α production and NF-κB p65 expression at 1 μmol/L and 10 μmol/L concentrations. Furthermore, there was no significantly difference between 1 μmol/L and 10μmol/ L. Conclusions LPS activated NF-κB and induced the production of TNF-α in cultured human monocytes. DXM could inhibit the activation of NF-κB and reduce the release of TNF-α in cultured human monocytes. This suppressive effect may not be related to its dosage.
出处
《中华急诊医学杂志》
CAS
CSCD
2007年第3期251-254,共4页
Chinese Journal of Emergency Medicine
基金
湖北省自然科学基金资助项目（2002ABl47）
