摘要
目的:研究松潘乌头总碱(TAS)的镇痛作用机制。方法:采用小鼠热板法和大鼠甩尾法测定动物的痛阈,同时检测乙酸致痛小鼠脑组织NO、MDA含量及SOD活性。结果:icv TAS0.025mg/kg能明显提高小鼠、大鼠的痛阈;TAS能使乙酸致痛小鼠脑组织NO、MDA含量明显降低,SOD活性明显增强;CaCl2能拮抗TAS的镇痛作用,Ver则使之增强。结论:TAS的镇痛作用部位可能在中枢神经系统,其作用机制可能与影响Ca2+内流和减少NO、MDA含量,提高SOD活性有关。
Objective ：To study the mechanism of analgesic action of total alkaloids of Aconitum sungpanense Hand-Mazz （TAS）. Methods：The pain threshold in mice and rats was determined by using analgesic assays of hot plate in mice and tail flicking in rats. To determine the nitric oxide（NO） ,the malondialdehyde （MDA） contemts ,superoxide dismutase（SOD） activity in the brain tissue of acetic acid writhing mouse. Resuits.The pain threshold of mice and rats was elevated after icy TAS in a dose of 1/10 systemic effective dose （0. 025mg/kg）. TAS（0.5,0. 25mg/kg, sc） could obvioualy lowered contents of NO and MDA, and increased SOD activity in the brain tissue of acetic acid writhing mouse. The analgesic action of TAS could be antagenized by CaCl2 and enhanced by verapamil. Conclusion ： The site of analgesic action of TAS may be in the central nervous system. Its mechanism of analgesic action are relate to its influence on the transmembrace influx of Ca^2 ＋ and reducing the output NO and removing free radicals.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第1期36-37,共2页
Pharmacology and Clinics of Chinese Materia Medica
关键词
松潘乌头总碱
镇痛作用
CA^2+
内流
NO
MDA
SOD
total alkaloids of Aconitum sungpanense Hand-Mazz.
analgesic action
Ca^2＋ influx
nitre oxide （NO）
superoxide dismutase （SOD）
malondialdehyde （MDA）.
