摘要
目的:探讨通络冻干粉针中有效成分栀子苷和芍药苷的药代动力学。方法:Beagle犬以高、中、低三个剂量分别单剂量静注给药,采用HPLC法测定不同时间Beagle犬体内栀子苷和芍药苷的血药浓度,以药动学计算软件对血药浓度-时间数据进行拟合。结果:高、中、低三种剂量分别单剂量静注给药后栀子苷、芍药苷在Beagle犬体内过程均呈二室模型,栀子苷高、中、低剂量组消除半衰期分别为113.5±41.5min,120.1±39.4min,161.0±22.6min,芍药苷为106.2±46.4min,132.7±46.8min,152.0±78.0min。结论:所建立的方法准确、灵敏度高,专属性好,可用于栀子苷和芍药苷的体内血浆药物浓度的同时分析。
Objective： A HPLC method was established for determination of genioside and paeoniflorin in plasma after iv Tongluo injection to dogs. Methods ： After iv beagle dogs with high dosage, middle dosage, low dosage singly, HPLC was used to determine genioside and paeoniflorin in dogs＇ plasma at different times, and a pharmacokinetic computable procedure was used to process concentratoion-time data. Results ： Beagle dogs were given high dosage, middle dosage, low dasage singly, and an open two-compartment model bes described the concentration time profile for genioside and paeoniflorin. The halflives of geinoside after i.v. high dosage, middle dosage and low dosage singly were 113.5 ± 41.5min, 120.1 ± 39.4min, 161.0 ± 22.6min, the halflives of paeoniflorin were 106.2 ± 46.4min, 132.7 ± 46.7min, 152.0 ± 78.0min. Conclusion ： The analytical method was shown to be an accurate and sensitive way in detecting the in vivo blood concentration of genioside and paeoniflorin in plasma at the same time.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第1期50-54,共5页
Pharmacology and Clinics of Chinese Materia Medica
关键词
通络冻干粉针
栀子苷
芍药苷
药代动力学
血药浓度
Tongluo Freeze-dried powder injection（通络冻干粉针） , genioside, paeoniflorin, HPLC, pharmacokinetics
