摘要
对大港油田的不同油样进行富集培养，分离到2株可将菲作为惟一碳源和能源的细菌菌株S17和S28，其可降解温度为20-65℃，最适宜降解温度为37℃。经16S rDNA序列同源性分析，两者属于假单孢菌属的不同种。菌株S17与食树脂假单孢茵（Pseudomonas resinovorans）的序列同源性为97％；菌株S28与高温假单胞菌（Pseudomonas thermaerum）的序列同源性达到100％，初步推测S28为高温假单胞菌。将两者用于原油的降解，7d后2株菌对原油的降解率分别达到74．7％和80．7％，经原油族组分分析，2株菌都可降解原油中的重烃组分。外加碳源蔗糖和氮源酵母膏可在很大程度上促进这2株菌对原油的降解。岩心实验结果表明。2株菌的混合发酵液可提高聚合物驱后岩心的原油采收率12．26％。
Two bacterial species S17 and S28 have been isolated from different oil samples of Dagang Oilfield, whose unique carbon and energy sources could be phenanthrene. The species can degrade phenanthrene between 20-65℃. The optimum temperature is 37℃. The two bacteria are identified as different strains of Pseudomonas genus on the basis of biochemical tests and 16SrDNA sequences assay. The homology is 97% between the species S17 and Pseudomonas resinovorans, and the homology is 100% between the species S28 and Pseudomonas thermaerum, therefore,the species S28 is assumed to be Pseudomonas thermaerum. They are used to degrade crude oil and the degradation rates are respectively 74.7% and 80.7% after 7 days' degradation. According to the analysis of content and group composition of the crude oil, both of them have the ability to degrade the heavy hydrocarbons in the crude oil. The addition of cane sugar as a carbon source and microzyme as a nitrogen source can enhance the degradation rate of the crude oil widely. The results of core experiments show that the mixture fermentation liquid of these two bacteria can increase the recovery factor of the crude oil after polymer flooding by 12.26%.
出处
《油气地质与采收率》
CAS
CSCD
北大核心
2007年第2期99-101,共3页
Petroleum Geology and Recovery Efficiency
基金
国家自然科学基金(40472152)
湖北省科技攻关项目(2004AA101C55)
关键词
多环芳烃
菲
微生物降解
MEOR
polycyclic aromatic hydrocarbons, phenanthrene, biodegradation, MEOR
