摘要
目的探讨氟对成骨细胞细胞核因子κB受体活化因子配体（RANKL）mRNA表达的影响。方法分离小鼠乳鼠成骨细胞，取第3代成骨细胞，分别培养于含有矿化液（维生素C50mg／L、β-甘油磷酸钠10mmol／L、地塞米松10^-7md／L）和不含矿化液的L-DMEM培养基中，按染氟剂量[F-终剂量为0（对照组）、1、10、100、1000、10000、20000μg／L]分组，染氟72h后，提取细胞总RNA，通过反转录一聚合酶链反应（RT-PCR）方法，观察含矿化液和不含矿化液情况下培养的成骨细胞在不同染氟剂量下RANKLmRNA表达的变化。结果与对照组比较，非矿化染氟（不含矿化液）1000μg/L组RANKLmRNA表达升高（P〈0．01），10000μg／L组RANKLmRNA表达降低（P〈0．01）；与对照组比较，矿化染氟100、1000μg／L组RANKLmRNA表达升高（P〈0.01）；相同染氟剂量，矿化染氟0、1、10、100、1000、10000μg／L组与非矿化染氟组比较，RANKLmRNA表达降低（P〈0．01）。结论染氟可以影响成骨细胞表达RANKLmRNA；同等染氟剂量下，矿化处理可降低成骨细胞RANKLmRNA表达。
Objective To study the influence of fluoride on expression of receptor activator of NF-κB ligand（RANKL ） mRNA of osteoblasts. Methods Osteoblasts were isolated from calvaria of neonatal mouse and then either cultured in osteogenic medium （L-DMEM medium containing of dexamethasone 10^-7 mol/L, vitamin C 50 mg/L and sodium glycerophosphate 10 mmol/L） or not, and fluoride was added in a series of concentrations（0, 1, 10, 100, 1 000, 10 000 and 20 000 μ/L）. The expressions of osteoblastic RANKL mRNA were detected by RT-PCR. Results When osteoblasts were treated by fluoride alone, the expressions of RANKL mRNA increased at fluoride concentration of 1 000 μg/L and lowered at 10 000 μg/L, respectively. When osteoblasts were treated by fluoride in osteogenic medium, the expressions of RANKL mRNA increased at fluoride concentrations of 100 and 1 000 μg/L. At same fluoride concentration, the level of RANKL mRNA of. osteoblasts cultured in osteogenic medium was lower than that without osteogenic medium. Conclusions Fluoride affects RANKL mRNA expressions of osteoblasts, which can be lowered by osteogenic medium at same concentrations of fluoride.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第2期130-132,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金资助项目（30271156）
