摘要
建立了大鼠血浆中原儿茶酸含量测定的高效液相色谱方法。采用的色谱柱为DiamondsilTMC18柱(150mm×4.6mm,5μm);流动相为乙腈-水(体积比为9∶91,用H3PO4调pH至2.5);流速1.2mL/min;检测波长260nm;内标为对羟基苯甲酸。原儿茶酸的线性范围为0.050-3.20mg/L,线性相关系数为0.997 8,最低定量限为0.050mg/L,日内和日间测定的精密度(以相对标准偏差表示)均低于7.0%,准确度(以相对误差表示)为-1.4%-2.6%;在0.050,0.40,3.20mg/L低、中、高3个添加浓度水平下,血浆样品的提取回收率分别为83.4%,87.3%,91.1%。该方法简便,灵敏,准确,适用于大鼠体内原儿茶酸的药物动力学研究。
A method was developed for the quantitative determination of protocatechuic acid in rat plasma by high performance liquid chromatography （HPLC）. With added p-hydroxybenzoic acid as internal standard, the plasma samples were extracted with a solvent mixture of methanol and acetonitrile （ 1 ： 5, v/v）. The analyte was determined by HPLC with a Diamondsil^TM C18 column and a mobile phase of acetonitrile-water （adjusted to pH 2.5 with H3PO4 9： 91, v/v） at a flow rate of 1.2 mL/min. The UV detection wavelength was 260 nm. The assay exhibited a good linearity in the concentration range from 0. 050 to 3.20 mg/L with a correlation coefficient of 0. 997 8. The limit of quantification was 0. 050 mg/L. The relative standard deviations of intra-day and inter-day determination were both less than 7.0% and the accuracy ranged from - 1.4% to 2.6%. The extraction recoveries of protocatechuic acid from rat plasma samples at three concentration levels were 83.4%, 87. 396, and 91.1%, respectively. The developed method was applied to a pharmacokinetic study. The main pharmacokinetic parameters in rats after a single oral dose of 10 mL/kg body weight were as follows： Cmax of （3.16 ±0.03） μg/mL, tmax of （0.50 ± 0.00） h, AUC0→t of （39.9±5.9） μg · mL^-1·h, AUC0→∞ of （54.8±8.1）μg · mL^-1· h and t1/2 of （ 3.87±0.25 ） h. The method was proved to be simple, sensitive and accurate, thus suitable for the pharmacokinetic study of protocatechuic acid in rat plasma.
出处
《色谱》
CAS
CSCD
北大核心
2007年第2期207-210,共4页
Chinese Journal of Chromatography
关键词
高效液相色谱法
原儿茶酸
大鼠血浆
high performance liquid chromatography （ HPLC ）
protocatechuic acid
rat plasma
