摘要
目的本研究观察糖皮质激素吸入对急性发作期哮喘患者外周血CD4+T细胞Foxp3的影响。方法经支气管激发试验确诊的咳嗽变异性哮喘患者12例,予以辅舒酮吸入剂吸入治疗,分别于治疗前及治疗半年后测定其引起FEV1降低20%的乙酰甲胆碱浓度(PC20-FEV1)。采集外周血,以流式细胞仪方法检测CD4+CD25+Foxp3+T细胞的比例,并分离外周血单个核细胞,提取其总RNA,以RT-PCR方法检测其Foxp3 mRNA的表达。结果咳嗽变异性哮喘患者外周血中CD4+Foxp3+T细胞的比例显著低于健康对照者。经辅舒酮吸入治疗半年后气道反应性逐渐下降,外周血中CD4+CD25+Foxp3+T细胞比例显著增加,Foxp3 mRNA表达亦逐渐增加,且与气道反应性呈显著负相关。结论辅舒酮可有效降低咳嗽变异性哮喘患者气道高反应性,且此变化与CD4+CD25+Foxp3+T细胞增加,Foxp3 mRNA表达增强相关。
Objective To investigate the changes of regulatory T cells in cough variant asthma（CVA） and the effect of inhaled corticoids on the expression of Foxp3. Methods CVA patients were treated with inhaled fluticasone propionate for 6 months. Before and after treatment, the provocative concentration of methacholine causing a 20% fall in forced expiratory volume in one second （PC20-FEV1） was tested, and the percentage of CD4^＋ CD25^＋ Foxp3^＋ T cells in periphery blood CD4^＋ T cells, and the expression of Foxp3 mRNA were examined with flow cytometry and RT-PCR respectively. Results The percentage of CD4^＋ CD25^＋ Foxp3^＋ T cells and expression of Foxp3 mRNA in CVA patients was lower than that in the healthy individuals, and the treatment with ICS increased the percentage of CD4^＋ CD25^＋ Foxp3^＋ T ceils and expression of Foxp3 mRNA, together with elevated PC20-FEV1. Conclusion ICS can successfully decrease bronchial hyperreactivity, and this effects may partially due to the induction of CD4^＋ CD25^＋ Foxp3^＋ Tcells.
出处
《重庆医学》
CAS
CSCD
2007年第6期511-513,共3页
Chongqing medicine
