摘要
采用不同的提取方法和合成转化方法,建立了黄芪提取物及半合成产物的高效液相(HPLC)指纹图谱。黄芪提取物的HPLC检测条件为:Apollo-C18(150×4.6 mm,5μm)色谱柱,柱温40℃,流动相为乙腈:水(1:2),检测波长为205nm,流速为1.0 mL/min,进样量为10μl。结果表明:碱提法黄芪甲苷分离效果最好,由低到高的顺序为:水提法<醇提法<超声波法<碱提法;在2%硫酸溶液酸化条件下半合成产物的指纹图谱最好。建立的指纹图谱测定方法,适用于黄芪提取物及半合成产物的质量控制。
Objective： The fingerprints of Radix astragali extraction and semi-synthesis product with different extracting and synthesis methods were established by high-performance liquid chromatography. Methods： The chromatographic condition： column： Apollo-C18 （150 4. 6 mm, 5 μm）, oven temperature： 40℃, mobile phase： Acetonitrile-water（33. 3： 66. 7v/v）, UV detection wavelength： 205 nm, flow rate： 1.0 mL min-1 , injection volume： 10μl. Results： The alkali-extracting and semi-synthesis product treated with 2% sulfuric acid showed the best fingerprints. Conclusion： The fingerprints of Radix astragali and semi-synthesis product were established with high stability and good reproducibility, and this method could be used in quality control.
出处
《海洋湖沼通报》
CSCD
北大核心
2007年第1期38-43,共6页
Transactions of Oceanology and Limnology
