摘要
目的研究感染性腹泻患者粪便中分离的阴沟肠杆菌携带的小肠结肠炎耶尔森菌HPI毒力岛irp-2基因,并评价其菌株的毒力和耐药情况。方法PCR扩增法检测毒力岛irp-2基因,小白鼠腹腔注射法检测毒力,药敏试验采用K-B纸片扩散法。结果从感染性腹泻患者粪便中分离的9株阴沟肠杆菌均扩增出irp-2基因片段,其相对分子量与对照菌株小肠结肠炎耶尔森菌WA株(O8型)的相应PCR片段一致,并具有较强的毒力。结论检出的阴沟肠杆菌含有小肠结肠炎耶尔森菌HPI毒力岛irp-2基因且为具有毒力的菌株,与致病性密切相关。
Objectlve To investigate if there is HIP virulent irp^-2 gene of Yersinia Yersinia enterocolitica in Enterobacter cloacae isolated from stool samples of infective diarrhea patients in army soldiers of Yunnan Garrison. Methods HIP virulent irp^- 2 gene of Yersinia Yersinia enterocolitica was determined by PCR and drug resistance test was conducted with K - B diffusion method. Results There irp^- 2 gene fragments were obtained from 9 strains of Enterobacter cloacae in stool samples of infective diarrhea patients , the relative molecular weight was identical to the fragments amplified from WA strain （08 type ） Yersinia Yersinia enterocolitica in control group and showed strong virulence. Conclusion There is HIP virulent irp^-2 gene of Yersinia Yersinia enterocolitica in Enterobacter cloacae and it is a strong virulent strain associated with pathogenicity.
出处
《中国热带医学》
CAS
2007年第4期502-503,共2页
China Tropical Medicine
