摘要
基于100℃反应1.0min和pH=4.0HAC-NaAC缓冲溶液的条件下,NO_2^-与Griess试剂(对氨基苯磺酸,α-萘胺)反应生成红色偶氮化合物在固体基质上能发射强而稳定的室温燐光,Hg^(2+)能催化红色偶氮化合物致使其燐光激烈猝灭,且Hg^(2+)的含量与△Ip成正比,据此建立了红色偶氮化合物固体基质室温燐光猝灭法测定痕量Hg^(2+)的新方法.Hg2+的含量在0.0080～4.0(fg/spot)(对应浓度0.020～10.00pg/ml)范围内与△Ip符合比尔定律,检出限为4.1ag/spot(对应浓度1.0×10^(-14)g/ml),对0.0080和4.0(fg/spot)的Hg^(2+)进行8次平行测定,其RSD分别为3.6%与4.1%,本方法成功用于水中痕量Hg2+的测定.
A new method for the determination of trace mercury has been proposed. It based on the fact that in HAc-NaAc buffer solution （pH = 4.0）, NO2^- can react with Griess reagent （a-naphthylamine） to form red azo-compound at 100℃ for 1.0min, which can emit strong and stable solid substrate-room temperature phosphorescence （SS-RTP）. And. mercury（ Ⅱ ） can catalyze the red azo-compound and cause the sharp quenching of SS-RTP. The reducing value of phosphorescence intensity （Alp） is directly proportional to the concentration of mercury. Therefore, a new method for the determination of trace mercury by solid substrate-room temperature phosphorescence quenching method based on red azo-compound has been established. The linear range of this method is 0.0080 - 4.0 fg/spot （corresponding concentration：0.020-10.00 pg /ml） with a detection limit of 4.1 ag/spot（corresponding concentration： 1.0×10^-14 g/ml）. The regression equation can be expressed as Alp = 30.40 ＋29.50m Hg^2＋ （fg/spot）, with the correlation coefficient r = 0.9996 （n=5）. After eight fold measurement, RSD were 3.6% and 4.1% for the samples containing 0.0080 and 4.0 fg/spot of mercury （ Ⅱ ）, respectively. This method has been applied to the determination of trace mercury（ Ⅱ ） in water successfully.
出处
《漳州师范学院学报（自然科学版）》
2007年第1期94-98,共5页
Journal of ZhangZhou Teachers College（Natural Science)
关键词
汞
Gricss试剂
红色偶氮化合物固体基质室温烧光猝灭法
Mercury（ Ⅱ）
Griess reagent
Solid substrate-room temperature phosphorescence quenching method based on red azo-compound
