摘要
目的探讨非小细胞肺癌PTEN蛋白表达与细胞凋亡和增殖的关系及其临床病理意义。方法采用SABC免疫组化法，检测43例非小细胞肺癌及20例癌旁正常肺组织石蜡包埋标本PTEN蛋白和增殖细胞核抗原（PCNA）表达；用TUNEL法检测细胞凋亡，以增殖指数和凋亡指数表示增殖与凋亡。结果肺癌组织PTEN蛋白的表达显著低于癌旁正常肺组织（X^2=13．609，P＜0．01），PTEN蛋白的表达与肺癌病人的组织类型及临床分期无关，而与肺癌组织的分化程度、淋巴结转移以及病人的预后密切相关（X^2=5．310～8．503，P＜0．05、0．01）；肺癌组织细胞凋亡指数明显高于癌旁正常肺组织（t=8．279，P＜0．01），细胞凋亡与肺癌的组织类型、临床分期以及淋巴结转移无关，而与病人的预后及组织分化程度相关（t=2．827、3．114，P＜0．05、0．01）；肺癌组织细胞增殖指数明显高于癌旁正常肺组织（t=9．620，P＜0．01），细胞增殖与肺癌的组织类型、组织的分化程度以及淋巴结转移无关，而与病人的临床分期、预后密切相关（t=1．993、2．800，P＜0．05、0．01）；细胞凋亡指数随肿瘤PTEN的表达水平增高而增高，细胞增殖指数随PTEN表达水平增加而降低。结论PTEN具有诱导细胞凋亡和抑制细胞增殖的双重作用，肺癌PTEN蛋白的低表达及由此导致的细胞凋亡、增殖失衡在肺癌发生发展过程中起重要作用。
Objective To study the expression of PTEN and its relationship with cell proliferation and apoptosis in nonsmall-cell lung cancer （NSCLC）. Methods The immunohistochemistry stain （SABC） method was applied to investigate the expression of PTEN and PCNA in samples of 43 NSCLC and 20 normal adjacent lung tissues. TUNEL method was applied to detect the apoptosis. The apoptosis index （AI） and mitotic index （MI） were defined. Results The positive rate of PTEN was significantly lower in cancer tissues than in their normal adjacent tissue （X^2 = 13. 609, P〈0.01）. The expression of PTEN had close rela- tion with the differentiation, lymph node metastasis and prognosis of the disease （X^2 =8. 503,P〈0.01 ; X^2 =5. 310,P〈0.05; X^2 = 6. 568 ,P〈0.05）, but not related to TNM staging and histological type. The AI of diseased tissues was higher than that of the normal adjacent tissues （t=8. 279,P〈0.01）. No relation was found among AI and TNM staging, lymph node metastasis and tissue differentiation. AI had correlation with differentiation degree and prognosis of the cancer （t = 2. 827, P〈 0.05 ; t = 3. 114, P〈 0.01）. The MI of the cancer was higher than that of normal tissues adjacent （t=9. 620,P〈0.01）. There was no relationship between MI and differentiation degree, lymph node metastasis and histological differentiation. MI correlated with TNM staging and prognosis of the carcinoma （t= 2. 800,P〈0.01 ;t= 1. 993 ,P〈0.05）. The AI increased and the MI decreased with the increase of positive PTEN. Conclusion The low expression of PTEN in lung cancer results in apoptosis and disequilibrium of proliferation, inducing apoptosis and inhibiting proliferation, plays an important role in the occurrence and development of the disease.
出处
《青岛大学医学院学报》
CAS
2007年第1期8-11,14,共5页
Acta Academiae Medicinae Qingdao Universitatis
