摘要
目的探讨应用流式细胞术(FCM)进行念珠菌快速药敏试验的方法及其应用价值。方法应用FCM检测氟康唑(FCZ)对念珠菌的抗菌活性,药物作用时间3 h后,用碘化丙啶(PI)染色,分别检测10株念珠菌阴性对照管和M IC药物浓度作用管的平均荧光强度(MFI),计算两者比值,据此,设定流式细胞药敏试验(FCST)检测M IC的判断值,并根据该判断值对16株临床菌株同时进行常规药敏和FCST双盲测定。结果10株念珠菌测得的MFI比值为2.03,CV为6.9%;将MFI大于阴性对照管2倍(增加100%)的最低药物浓度定义为该菌的M IC;16株临床菌株中,有15株念珠菌两种方法测得M IC值相差在1个倍比稀释度以内,两者差异无统计学意义(P>0.05)。结论FCST与常规药敏试验结果有很好的一致性,并且具有快速、敏感、准确、便于自动化等优势。
Objective A rapid method for antifungal susceptibility testing was developed by Flow Cytometry （FCM） and its application value was studied by comparing with M27-A method. Methods The antifungal activity of fluconazole（FCZ） was detected by flow cytometry. The samples were incubated with FCZ in different concentrations for 3 hours, then they were stained by propidium iodide （PI）. To 10 Candida isolates, mean fluorescence intensity （MFI） of negative control and reaction tube for drug MIC was detected respectively. According to the judgement level of MIC by Flow cytometry susceptibility testing（ FCST）, 16 clinical isolates were double-blind examined at the same time by M27-A method and FCST. Results The mean of ratios for MFI of 10 Candida isolates was 2.03 ,and the coefficient of variation was 6.9% ; The MIC was defined as the lowest concentration of drug that showed an increase of 100% in MFI compared to that of the negative control. Comparing the MICs of FCST and M27-A method in 16 clinical isolates,the difference of MIC value for 15 isolates was within 1 dilution,there was no significant difference in MIC between FCST and M27-A method （P 〉 O. 05 ）. Conclusion The results obtained by FCST and M27-A method are in excellent agreement, and FCST is a rapid, accurate and sensitive and convenient method for automatization.
出处
《中国临床保健杂志》
CAS
2007年第2期127-130,共4页
Chinese Journal of Clinical Healthcare
基金
安徽省立医院院助课题资助项目(2005YZ06)
