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水貂源犬瘟热病毒F基因真核表达载体的构建及鉴定 被引量：1

Construction and Identification of Eukaryotic Expression Plasmid with Fusion Protein Gene of Canine Distemper Virus Isolated from Mink
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摘要 通过T-A克隆技术,成功地构建了克隆载体PMD-18T-CDVF,用KpnI和BamHI双酶切克隆质粒PMD-18T-CDVF后,回收F基因,并将此基因定向克隆至相同双酶切回收后的pcDNA3.1真核表达载体中,获得重组质粒pcDNA3.1-CDVF。经DNA测序、限制性内切酶分析和PCR鉴定,证实成功地构建了重组质粒,从而为研究犬瘟热新型疫苗奠定了基础。 The clone vector PMD-18T-CDVF with fusion gene of canine distemper virus isolated from a mink was successfully constructed by T- A clone technique and was digested with restriction enzyme of Kpn I and BamH I. The F gene was cloned into eukaryotic expression vectorpcDNA3.1. Finally a recombinant plasmid named pcDNA3.1-CDVF was constructed and identified by PCR, restriction enzyme analysis and sequencing.
作者 苏凤艳 全学俊 宗春苗 王卓聪 丁庆东 王全凯
机构地区 吉林农业大学中药材学院 图门市牧业管理局
出处 《经济动物学报》 CAS 2007年第1期23-26,共4页 Journal of Economic Animal
关键词 水貂 犬瘟热病毒 F基因 真核表达载体 mink canine distemper virus F gene eukaryotic expression vector
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献10

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二级参考文献16

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共引文献24

同被引文献10

  • 1闫芳,夏咸柱,李德生,王鹏彦,高凤山,高玉伟,黄耕.犬瘟热减毒疫苗对小熊猫安全性与免疫原性研究[J].病毒学报,2004,20(3):275-278. 被引量：10
  • 2陈武,陈洪汉,黄勉,张马龙.弱毒疫苗接种导致小熊猫犬瘟热病毒感染的诊断[J].黑龙江畜牧兽医,2006(1):76-78. 被引量：10
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引证文献1

二级引证文献9

经济动物学报
2007年 第1期

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