摘要
为了研究用柠檬酸铝、混合稀土或亚硒酸钠预处理温石棉对石棉诱导人胚肺(HEL)细胞产生羟基自由基(·OH)的影响,笔者用不同浓度的柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡温石棉1小时后,再将其与人胚肺细胞共同孵育,利用电子自旋捕获法测定反应体系中羟基自由基信号。结果显示,石棉可诱导人胚肺细胞产生羟基自由基,并且所产生的自由基信号(峰高)随石棉浓度升高而增强,呈一定的剂量依赖性。经3种化合物预处理的温石棉组人胚肺细胞所产生的自由基信号(峰高)均明显低于未处理温石棉组。上述结果表明,温石棉可诱导人胚肺细胞产生羟基自由基,但用3种化合物预处理温石棉,均可抑制温石棉诱导人胚肺细胞产生羟基自由基。
To study the effects of native chrysotile and compounds-pretreated chrysotile on the production of hydroxyl radical （^·OH）in human embryo lung （HEL） cells, the chrysotile, after being soaked in aluminium citrate, mixed rare earths or sodium selenite solutions at different concentrations for 1 hour, were incubated with HEL cells for 10 min. , then the levels of signal of ^·OH were determined by using electron spin resonance （ESR）. The results showed that the HEL cells were exposed to native chrysotile at 0.125, 0.25, 0.5, 1.0μg/ml respectively for 10 rain. , the levels of signal of ：OH increased compared with control, and showed a good dose-effect relationship. But less increase could be found when cells were exposed to compounds-pretreated chrysotile. These results indicted that chrysotile can induce production of ^·OH in HEL cells, while the pretreatment of chrysotile with aluminium citrate, mixed rare earths or sodium selenite solutions can inhibit chrysotile-induced production of ^·OH in HEL cells.
出处
《中国安全科学学报》
CAS
CSCD
2007年第2期134-138,共5页
China Safety Science Journal
关键词
温石棉
表面改性
人胚肺细胞
羟基自由基
chrysotile
surface modification
human embryo lung ceils
hydroxyl radical
