摘要
采用正交设计方法,研究了NAA、6-BA、MS培养基对明党参叶片与叶柄愈伤组织诱导的影响。结果表明,叶片和叶柄都可以诱导出愈伤组织,但叶片较叶柄更易诱导出愈伤组织;在不同激素配比的培养基中,以1/2 MS+NAA1.0 mg/L+6-BA2.0 mg/L的培养基激素组合对明党参愈伤组织诱导效果较好。
Effects of NAA, 6 -BA, MS culture medium on leaf and petiole callus induction of Changium smyrnioides Wolff were studied by orthogonal design. The results showed that leaf and petiole could induce callus, and leaf was easier to induce callus than petiole. The optimal medium for callus induction was 1/2 MS ＋ NAA 1.0 mg/L ＋ 6-BA 2.0 mg/L.
出处
《安徽农业科学》
CAS
北大核心
2007年第8期2232-2233,共2页
Journal of Anhui Agricultural Sciences
基金
江苏省药用植物生物技术重点实验室项目(KJS03077)
关键词
明党参
愈伤组织
正交设计
叶
Changium smyrnioides Wolff
Callus
Orthogonal design
Leaf
